Literature DB >> 6538892

Separation of viable microfilariae free of blood cells on Percoll gradients.

R Chandrashekar, U R Rao, G R Rajasekariah, D Subrahmanyam.   

Abstract

A consistent and reproducible method is described for isolating pure populations of microfilariae of Litomosoides carinii, Brugia pahangi, B. malayi and Dipetalonema viteae, free of cells, from blood, by density gradient centrifugation on Percoll in 0.25 M sucrose. The recovery of the microfilariae was 85 to 97%.

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Year:  1984        PMID： 6538892     DOI： 10.1017/s0022149x00028078

Source DB:  PubMed          Journal:  J Helminthol        ISSN： 0022-149X            Impact factor:   2.170


  22 in total

1.  Use of a simple DNA extraction method for high-throughput detection of filarial parasite Wuchereria bancrofti in the vector mosquitoes.

Authors:  V Vasuki; S Subramanian; S L Hoti; P Jambulingam
Journal:  Parasitol Res       Date:  2012-07-10       Impact factor: 2.289

2.  Effect of chemotherapeutic treatment on cytokine (IFN-gamma, IL-2, IL-4, IL-5, IL-10) gene transcription in response to specific antigens in Brugia malayi-infected Mastomys coucha.

Authors:  Mareike Saunders; Anja Taubert; Tarig Dafa'alla; Horst Zahner
Journal:  Parasitol Res       Date:  2008-08-05       Impact factor: 2.289

3.  The sheaths of Brugia microfilariae: isolation and composition.

Authors:  T Klonisch; G Bardehle; D Linder; B Boschek; H H Schott; H Zahner; S Stirm
Journal:  Parasitol Res       Date:  1991       Impact factor: 2.289

4.  Use of microarray hybridization to identify Brugia genes involved in mosquito infectivity.

Authors:  Kathryn G Griffiths; George F Mayhew; Rebecca L Zink; Sara M Erickson; Jeremy F Fuchs; Colleen M McDermott; Bruce M Christensen; Michelle L Michalski
Journal:  Parasitol Res       Date:  2009-11-06       Impact factor: 2.289

5.  IgG response of rats to the excretory-secretory products of Litomosoides carinii.

Authors:  R Chandrashekar; U R Rao; D Subrahmanyam
Journal:  Parasitol Res       Date:  1990       Impact factor: 2.289

6.  Isolation of pure sheaths of Litomosoides carinii microfilariae.

Authors:  G Bardehle; T Klonisch; H H Schott; S Stirm; H Zahner
Journal:  Parasitol Res       Date:  1987       Impact factor: 2.289

7.  Patency of Litomosoides sigmodontis infection depends on Toll-like receptor 4 whereas Toll-like receptor 2 signalling influences filarial-specific CD4(+) T-cell responses.

Authors:  Maria B Rodrigo; Sandy Schulz; Vanessa Krupp; Manuel Ritter; Katharina Wiszniewsky; Kathrin Arndts; Ruth S E Tamadaho; Elmar Endl; Achim Hoerauf; Laura E Layland
Journal:  Immunology       Date:  2016-04       Impact factor: 7.397

8.  Interleukin-4 is essential for the control of microfilariae in murine infection with the filaria Litomosoides sigmodontis.

Authors:  L Volkmann; M Saeftel; O Bain; K Fischer; B Fleischer; A Hoerauf
Journal:  Infect Immun       Date:  2001-05       Impact factor: 3.441

9.  Effect of ivermectin on filariae of Mastomys natalensis.

Authors:  U R Rao; R Chandrashekar; D Subrahmanyam
Journal:  Parasitol Res       Date:  1990       Impact factor: 2.289

10.  Formation by the uterus of a peripheral layer of the sheath in microfilariae of Litomosoides carinii and Brugia malayi.

Authors:  U Schraermeyer; W Peters; H Zahner
Journal:  Parasitol Res       Date:  1987       Impact factor: 2.289
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