Literature DB >> 6532239

Detection of glutathione transferase activity on polyacrylamide gels.

G Ricci, M Lo Bello, A M Caccuri, F Galiazzo, G Federici.   

Abstract

A simple and sensitive assay for glutathione transferase activity on polyacrylamide gel is described. The method is based on the fast reduction of nitroblue tetrazolium salt by glutathione. Blue insoluble formazan colors the gel except in the glutathione transferase area. The stable and defined colorless zone is still detectable with 0.005 unit enzyme. This technique has been successfully applied with enzyme preparations of human heart and other tissues.

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Year:  1984        PMID： 6532239     DOI： 10.1016/0003-2697(84)90657-2

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  14 in total

1.  Role of bacterioferritin comigratory protein and glutathione peroxidase-reductase system in promoting bentazone tolerance in a mutant of Synechococcus elongatus PCC7942.

Authors:  Palash Kumar Das; Suvendra Nath Bagchi
Journal:  Protoplasma       Date:  2011-01-27       Impact factor: 3.356

2.  Studies on glutathione S-transferases important for sperm function: evidence of catalytic activity-independent functions.

Authors:  B Gopalakrishnan; S Aravinda; C H Pawshe; S M Totey; S Nagpal; D M Salunke; C Shaha
Journal:  Biochem J       Date:  1998-01-15       Impact factor: 3.857

3.  Biochemical studies on glutathione S-transferase from the bovine filarial worm Setaria digitata.

Authors:  Lakshmy Srinivasan; Nisha Mathew; Twinkle Karunan; Kalyanasundaram Muthuswamy
Journal:  Parasitol Res       Date:  2011-01-05       Impact factor: 2.289

4.  H.p.l.c. separation and study of the charge isomers of human placental glutathione transferase.

Authors:  L L Radulovic; A P Kulkarni
Journal:  Biochem J       Date:  1986-10-01       Impact factor: 3.857

5.  Purification and biochemical characterization of cytosolic glutathione-S-transferase from malarial parasites Plasmodium yoelii.

Authors:  Rumana Ahmad; Arvind K Srivastava
Journal:  Parasitol Res       Date:  2006-10-06       Impact factor: 2.289

6.  Isolation and characterization of octopus hepatopancreatic glutathione S-transferase. Comparison of digestive gland enzyme with lens S-crystallin.

Authors:  S S Tang; C C Lin; G G Chang
Journal:  J Protein Chem       Date:  1994-10

7.  A bentazone-resistant mutant of cyanobacterium, Synechococcus elongatus PCC7942 adapts different strategies to counteract on bromoxynil- and salt-mediated oxidative stress.

Authors:  Suvendra Nath Bagchi; Palash Kumar Das; Sonali Banerjee; Mona Saggu; Divya Bagchi
Journal:  Physiol Mol Biol Plants       Date:  2012-03-31

8.  The role of antioxidant defense in freezing tolerance of resurrection plant Haberlea rhodopensis.

Authors:  Katya Georgieva; Gergana Mihailova; Liliana Gigova; Soleya Dagnon; Lyudmila Simova-Stoilova; Maya Velitchkova
Journal:  Physiol Mol Biol Plants       Date:  2021-05-07

9.  Long term effect of curcumin in restoration of tumour suppressor p53 and phase-II antioxidant enzymes via activation of Nrf2 signalling and modulation of inflammation in prevention of cancer.

Authors:  Laxmidhar Das; Manjula Vinayak
Journal:  PLoS One       Date:  2015-04-10       Impact factor: 3.240

10.  Effect of polyphenols extracted from Punica granatum and Acacia saligna plants on glutathione S-transferase of the cattle tick Rhipicephalus (Boophilus) annulatus (Acari: Ixodidae).

Authors:  Rasha A Guneidy; Mahmoud A Amer; Amr E El Hakim; Sobhy Abdel-Shafy; Shaimaa A Allam
Journal:  J Parasit Dis       Date:  2021-01-05
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