Literature DB >> 6530457

Protein conformation and reversed-phase high-performance liquid chromatography.

A J Sadler, R Micanovic, G E Katzenstein, R V Lewis, C R Middaugh.   

Abstract

The structure of a series of proteins has been investigated by circular dichroism, fluorescence and visible spectroscopy as well as by differential scanning calorimetry under reversed-phase high-performance liquid chromatography elution conditions. These studies show that 1-propanol, a typical eluent, induces a reversible conformational change in proteins to an apparently ordered, helical form. This structural transition occurs in the range of propanol concentrations that produces elution of a particular protein. The possible relationship between this conformational change and protein elution is considered.

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Year:  1984        PMID： 6530457     DOI： 10.1016/s0021-9673(01)91650-4

Source DB:  PubMed          Journal:  J Chromatogr


  3 in total

1.  Purification and characterization of ten new rice NaCl-soluble proteins: identification of four protein-synthesis inhibitors and two immunoglobulin-binding proteins.

Authors:  G G Limas; M Salinas; I Moneo; S Fischer; B Wittmann-Liebold; E Méndez
Journal:  Planta       Date:  1990-04       Impact factor: 4.116

2.  Utility of cleavable isotope-coded affinity-tagged reagents for quantification of low-copy proteins induced by methylprednisolone using liquid chromatography/tandem mass spectrometry.

Authors:  Jun Qu; William J Jusko; Robert M Straubinger
Journal:  Anal Chem       Date:  2006-07-01       Impact factor: 6.986

3.  Role of conformational changes in the elution of proteins from reversed-phase HPLC columns.

Authors:  G E Katzenstein; S A Vrona; R J Wechsler; B L Steadman; R V Lewis; C R Middaugh
Journal:  Proc Natl Acad Sci U S A       Date:  1986-06       Impact factor: 11.205
  3 in total

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