The use of liquid chromatography with dual-electrode electrochemical detection in the investigation of glutathione oxidation during benzene metabolism.

Liquid chromatography with electrochemical detection provides a powerful tool for the study of glutathione oxidation during benzene metabolism. The use of two dual-electrode detectors allows for the detection of oxidized and reduced glutathione and of phenol and quinone metabolites of benzene. The role of glutathione as a reductant is explored in this paper. Results indicate that hydrogen peroxide is the oxidizing agent primarily responsible for glutathione oxidation during benzene, phenol and hydroquinone metabolism.