Literature DB >> 6530144

Cloning gene ura5 for the orotidylic acid pyrophosphorylase of the filamentous fungus Podospora anserina: transformation of protoplasts.

J Bégueret, V Razanamparany, M Perrot, C Barreau.   

Abstract

From a genomic library of the filamentous fungus Podospora anserina, we have cloned a 4.9-kb fragment which complements an Escherichia coli mutant strain deficient for orotidylic acid pyrophosphorylase (pyrE gene). The recombinant plasmid pPAura5 also transforms to prototrophy a mutant strain of P. anserina carrying a mutation in the ura5 gene and lacking OMPppase activity.

Entities:  

Mesh：

Substances：

Year:  1984        PMID： 6530144     DOI： 10.1016/0378-1119(84)90023-4

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  22 in total

1.  Intronic GIY-YIG endonuclease gene in the mitochondrial genome of Podospora curvicolla: evidence for mobility.

Authors:  C Saguez; G Lecellier; F Koll
Journal:  Nucleic Acids Res       Date:  2000-03-15       Impact factor: 16.971

2.  Integrative transformation of the ascomycete Podospora anserina: identification of the mating-type locus on chromosome VII of electrophoretically separated chromosomes.

Authors:  H D Osiewacz; A Skaletz; K Esser
Journal:  Appl Microbiol Biotechnol       Date:  1991-04       Impact factor: 4.813

3.  Cloning the mating types of the heterothallic fungus Podospora anserina: developmental features of haploid transformants carrying both mating types.

Authors:  M Picard; R Debuchy; E Coppin
Journal:  Genetics       Date:  1991-07       Impact factor: 4.562

4.  Molecular cloning of the human UMP synthase gene and characterization of point mutations in two hereditary orotic aciduria families.

Authors:  M Suchi; H Mizuno; Y Kawai; T Tsuboi; S Sumi; K Okajima; M E Hodgson; H Ogawa; Y Wada
Journal:  Am J Hum Genet       Date:  1997-03       Impact factor: 11.025

5.  Transformation by integration in Podospora anserina. III. Replacement of a chromosome segment by a two-step process.

Authors:  E Coppin-Raynal; M Picard; S Arnaise
Journal:  Mol Gen Genet       Date:  1989-10

6.  Positive screening and transformation of ura5 mutants in the fungus Podospora anserina: characterization of the transformants.

Authors:  V Razanamparany; J Bégueret
Journal:  Curr Genet       Date:  1986       Impact factor: 3.886

Review 7.  The Thom Award address. Industrial mycology and the new genetics.

Authors:  P A Lemke
Journal:  J Ind Microbiol       Date:  1995-05

8.  Isolation and transformation of uracil auxotrophs of the lignin-degrading basidiomycete Phanerochaete chrysosporium.

Authors:  L Akileswaran; M Alic; E K Clark; J L Hornick; M H Gold
Journal:  Curr Genet       Date:  1993       Impact factor: 3.886

9.  Isolation of telomeric DNA from the filamentous fungus Podospora anserina and construction of a self-replicating linear plasmid showing high transformation frequency.

Authors:  J P Javerzat; V Bhattacherjee; C Barreau
Journal:  Nucleic Acids Res       Date:  1993-02-11       Impact factor: 16.971

10.  Assignment of linkage groups to the electrophoretically-separated chromosomes of the fungus Podospora anserina.

Authors:  J P Javerzat; C Jacquier; C Barreau
Journal:  Curr Genet       Date:  1993-09       Impact factor: 3.886
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.