Literature DB >> 6527553

Transcardial perfusion fixation of the CNS by means of a compressed-air-driven device.

B W Jonkers, J C Sterk, F G Wouterlood.   

Abstract

In the present report we describe the construction of a systemic vascular perfusion device designed to perfuse small mammals with fixatives under a constant, controlled hydrostatic pressure. The device is powered by compressed-air. The construction of the device allows the interchange of vessels containing various perfusates during the perfusion, without having to interrupt the flow or alter the hydrostatic pressure. Large volumes of fixatives can be perfused in a relatively short time, leading to good preservation of brain tissue, both for light and electron microscopical purposes.

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Year:  1984        PMID： 6527553     DOI： 10.1016/0165-0270(84)90013-x

Source DB:  PubMed          Journal:  J Neurosci Methods        ISSN： 0165-0270            Impact factor:   2.390


  7 in total

1.  Basic surgical techniques in the Göttingen minipig: intubation, bladder catheterization, femoral vessel catheterization, and transcardial perfusion.

Authors:  Kaare S Ettrup; Andreas N Glud; Dariusz Orlowski; Lise M Fitting; Kaare Meier; Jens Christian Soerensen; Carsten R Bjarkam; Aage K Olsen Alstrup
Journal:  J Vis Exp       Date:  2011-06-26       Impact factor: 1.355

2.  Anterograde neuroanatomical tracing with Phaseolus vulgaris-leucoagglutinin combined with immunocytochemistry of gamma-amino butyric acid, choline acetyltransferase or serotonin.

Authors:  F G Wouterlood
Journal:  Histochemistry       Date:  1988

3.  Dissection and Coronal Slice Preparation of Developing Mouse Pituitary Gland.

Authors:  Dongmei Cao; Xianhua Ma; Weiping J Zhang; Zhifang Xie
Journal:  J Vis Exp       Date:  2017-11-16       Impact factor: 1.355

4.  Disruption of Locomotion in Response to Hindlimb Muscle Stretch at Acute and Chronic Time Points after a Spinal Cord Injury in Rats.

Authors:  Anastasia V P Keller; Grace Wainwright; Alice Shum-Siu; Daniella Prince; Alyssa Hoeper; Emily Martin; David S K Magnuson
Journal:  J Neurotrauma       Date:  2016-08-23       Impact factor: 5.269

5.  Single-cell phenotyping within transparent intact tissue through whole-body clearing.

Authors:  Bin Yang; Jennifer B Treweek; Rajan P Kulkarni; Benjamin E Deverman; Chun-Kan Chen; Eric Lubeck; Sheel Shah; Long Cai; Viviana Gradinaru
Journal:  Cell       Date:  2014-07-31       Impact factor: 41.582

6.  Whole animal perfusion fixation for rodents.

Authors:  Gregory J Gage; Daryl R Kipke; William Shain
Journal:  J Vis Exp       Date:  2012-07-30       Impact factor: 1.355

7.  Electromyographic patterns of the rat hindlimb in response to muscle stretch after spinal cord injury.

Authors:  Anastasia V Keller; Kathlene M Rees; Erik J Seibt; B Danni Wood; Abigail D Wade; Johnny Morehouse; Alice Shum-Siu; David S K Magnuson
Journal:  Spinal Cord       Date:  2018-02-19       Impact factor: 2.772
  7 in total

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