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Literature DB >> 6520329

Uniformly spaced banding pattern in DNA sequencing gels by use of field-strength gradient.

Abstract

A method is described for casting and operating ultrathin wedge-shaped field-strength gradient gels for DNA sequencing. One of the gradients results in a uniformly-spaced oligonucleotide banding pattern from 60 to 300 nucleotides in a 53 cm long 4% polyacrylamide gel. From these field-strength gradient gels, more sequencing data can be obtained with greater confidence than from gels with uniform thickness. The gel-casting method is fast and simple. At 2.5 kV two gels can be run simultaneously in 2 h with the same power supply.

Entities: Chemical

Mesh：

Substances：
DNA, Bacterial

Year: 1984 PMID： 6520329 DOI： 10.1016/0165-022x(84)90053-8

Source DB: PubMed Journal: J Biochem Biophys Methods ISSN： 0165-022X

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Cited

15 in total

1. Genetic and sequence organization of the mcrBC locus of Escherichia coli K-12.

Authors: D Dila; E Sutherland; L Moran; B Slatko; E A Raleigh
Journal: J Bacteriol Date: 1990-09 Impact factor: 3.490

2. Direct solid phase sequencing of genomic and plasmid DNA using magnetic beads as solid support.

Authors: T Hultman; S Ståhl; E Hornes; M Uhlén
Journal: Nucleic Acids Res Date: 1989-07-11 Impact factor: 16.971

3. Rapid separation of large DNA fragments.

Authors: S E Peltek; L L Frumin; V V Chasovskikh
Journal: Dokl Biochem Biophys Date: 2005 Jul-Aug Impact factor: 0.788

4. Characterization of the cloned BamHI restriction modification system: its nucleotide sequence, properties of the methylase, and expression in heterologous hosts.

Authors: J E Brooks; P D Nathan; D Landry; L A Sznyter; P Waite-Rees; C L Ives; L S Moran; B E Slatko; J S Benner
Journal: Nucleic Acids Res Date: 1991-02-25 Impact factor: 16.971

9. Molecular characterization of the cysJIH promoters of Salmonella typhimurium and Escherichia coli: regulation by cysB protein and N-acetyl-L-serine.

Authors: J Ostrowski; N M Kredich
Journal: J Bacteriol Date: 1989-01 Impact factor: 3.490

10. Nucleotide sequence and transcriptional analysis of the redD locus of Streptomyces coelicolor A3(2).

Authors: K E Narva; J S Feitelson
Journal: J Bacteriol Date: 1990-01 Impact factor: 3.490

Uniformly spaced banding pattern in DNA sequencing gels by use of field-strength gradient.

1. Genetic and sequence organization of the mcrBC locus of Escherichia coli K-12.

2. Direct solid phase sequencing of genomic and plasmid DNA using magnetic beads as solid support.

3. Rapid separation of large DNA fragments.

4. Characterization of the cloned BamHI restriction modification system: its nucleotide sequence, properties of the methylase, and expression in heterologous hosts.

5. Structure and expression of the cytochrome aa3 regulatory gene ctaA of Bacillus subtilis.

6. Cloning the BamHI restriction modification system.

7. Nucleotide sequence of the DdeI restriction-modification system and characterization of the methylase protein.

8. Cloning, sequencing and expression of the Taq I restriction-modification system.

9. Molecular characterization of the cysJIH promoters of Salmonella typhimurium and Escherichia coli: regulation by cysB protein and N-acetyl-L-serine.

10. Nucleotide sequence and transcriptional analysis of the redD locus of Streptomyces coelicolor A3(2).