Literature DB >> 6520023

The Giemsa-11 technique for species-specific chromosome differentiation. A simple stain modification leading to dependable direct and sequential staining procedures.

C H Buys, G H Aanstoot, A J Nienhaus.   

Abstract

Oxidizing Methylene Blue and adding the reaction products to Eosin Y and Azure B makes possible a highly reliable Giemsa-11 technique for discrimination of chromosomes in hybrid cells according to their parental origin. This staining can be combined in a sequential procedure with a fluorescent banding technique allowing the exact identification of the chromosomes.

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Year:  1984        PMID： 6520023     DOI： 10.1007/bf00489751

Source DB:  PubMed          Journal:  Histochemistry        ISSN： 0301-5564


  10 in total

1.  Differential staining of human and mouse chromosomes in interspecific cell hybrids.

Authors:  M Bobrow; J Cross
Journal:  Nature       Date:  1974-09-06       Impact factor: 49.962

2.  Mechanisms involved in the banding of chromosomes with guinacrine and Giems. II. The interaction of the dyes with the chromosomal components.

Authors:  A T Sumner; H J Evans
Journal:  Exp Cell Res       Date:  1973-09       Impact factor: 3.905

3.  Staining of some specific regions of human chromosomes, particularly the secondary constriction of No. 9.

Authors:  M Bobrow; K Madan; P L Pearson
Journal:  Nat New Biol       Date:  1972-07-26

4.  Specific cytological recognition of the heterochromatic segment of number 9 chromosome in man.

Authors:  R Gagné; C Laberge
Journal:  Exp Cell Res       Date:  1972-07       Impact factor: 3.905

5.  Somatic cell genetics and gene mapping.

Authors:  F T Kao
Journal:  Int Rev Cytol       Date:  1983

Review 6.  New approaches to the study of chromosome organization.

Authors:  A T Sumner
Journal:  Sci Prog       Date:  1983       Impact factor: 2.774

7.  Somatic cell genetics and gene families.

Authors:  P D'Eustachio; F H Ruddle
Journal:  Science       Date:  1983-05-27       Impact factor: 47.728

8.  Simultaneous identification of chromatid replication and of human chromosomes in metaphases of man-mouse somatic cell hybrids. (With 1 color plate).

Authors:  B Alhadeff; M Velivasakis; M Siniscalco
Journal:  Cytogenet Cell Genet       Date:  1977

Review 9.  Somatic cell genetics and flow cytometry.

Authors:  M E Kamarck; J A Barbosa; L Kühn; P G Peters; L Shulman; F H Ruddle
Journal:  Cytometry       Date:  1983-09

10.  Reverse fluorescent chromosome banding with chromomycin and DAPI.

Authors:  D Schweizer
Journal:  Chromosoma       Date:  1976-11-29       Impact factor: 4.316
  10 in total
  3 in total

1.  Assignment of the gene(s) involved in the expression of the proliferation-related Ki-67 antigen to human chromosome 10.

Authors:  D M Schonk; H J Kuijpers; E van Drunen; C H van Dalen; A H Geurts van Kessel; R Verheijen; F C Ramaekers
Journal:  Hum Genet       Date:  1989-10       Impact factor: 4.132

2.  A straightforward approach to isolate DNA sequences with potential linkage to the retinoblastoma locus.

Authors:  H Scheffer; D van der Lelie; G H Aanstoot; N Goor; A J Nienhaus; A H van der Hout; P L Pearson; C H Buys
Journal:  Hum Genet       Date:  1986-11       Impact factor: 4.132

3.  Hybrid cell lines constitute a potential reservoir of polarized cells: isolation and study of highly differentiated hepatoma-derived hybrid cells able to form functional bile canaliculi in vitro.

Authors:  D Cassio; C Hamon-Benais; M Guérin; O Lecoq
Journal:  J Cell Biol       Date:  1991-12       Impact factor: 10.539
  3 in total

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