Literature DB >> 6517393

A method for freezing bovine lymphocytes.

R A Oliver, A J Springbett, R L Spooner.   

Abstract

A simple two-stage technique for preserving bovine lymphocytes is described. Lymphocytes from animals chosen at random were used. The experiments indicate that the optimum temperature for freezing and the optimum concentration of dimethylsulphoxide (DMSO) as cryoprotectant were in the range -29 degrees C to -31 degrees C and 17.5% to 20% respectively. These concentrations of DMSO are much greater than those reported in most other studies.

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Year:  1984        PMID: 6517393     DOI: 10.1111/j.1365-2052.1984.tb01118.x

Source DB:  PubMed          Journal:  Anim Blood Groups Biochem Genet        ISSN: 0003-3480


  1 in total

1.  Cryopreservation of Theileria-infected lymphoblastoid cells with functional assessment of viability.

Authors:  J M Wathanga; T W Jones; C G Brown
Journal:  Trop Anim Health Prod       Date:  1986-11       Impact factor: 1.559

  1 in total

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