Literature DB >> 6510503

Zone immunoelectrophoresis assay applied to alpha 1-acid glycoprotein secretion by isolated rat hepatocytes.

M Appel, J Davy, G Durand, D Biou, J Feger, J Agneray.   

Abstract

A method for measuring proteins in low concentrations applying the zone immunoelectrophoresis assay is reported. The low detection limit makes it possible to measure alpha 1-acid glycoprotein in rat serum and also to quantify the secretion of this protein after concentration of the incubation media containing less than 10(7) isolated rat hepatocytes. The method is simple and consumes very small quantities of antiserum.

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Year:  1984        PMID: 6510503     DOI: 10.1007/bf01951935

Source DB:  PubMed          Journal:  Experientia        ISSN: 0014-4754


  6 in total

1.  Immunochemical quantitation of antigens by single radial immunodiffusion.

Authors:  G Mancini; A O Carbonara; J F Heremans
Journal:  Immunochemistry       Date:  1965-09

2.  [Isolated hepatocytes fixed on collagen, an accurate model for the the secretion of proteins in a minimal medium. Response to inflammation].

Authors:  J Davy; M Appel; D Biou; J Feger; J Agneray
Journal:  Biol Cell       Date:  1983       Impact factor: 4.458

3.  Quantification of proteins with a new sensitive method--zone immunoelectrophoresis assay.

Authors:  O Vesterberg
Journal:  Hoppe Seylers Z Physiol Chem       Date:  1980-05

4.  Quantitative estimation of proteins by electrophoresis in agarose gel containing antibodies.

Authors:  C B Laurell
Journal:  Anal Biochem       Date:  1966-04       Impact factor: 3.365

5.  Rat alpha1-acid glycoprotein. Purification and immunological estimation of its serum concentration.

Authors:  K Shibata; H Okubo; H Ishibashi; K Tsuda
Journal:  Biochim Biophys Acta       Date:  1977-11-25

6.  High-yield preparation of isolated rat liver parenchymal cells: a biochemical and fine structural study.

Authors:  M N Berry; D S Friend
Journal:  J Cell Biol       Date:  1969-12       Impact factor: 10.539

  6 in total

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