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Literature DB >> 6509563

LR white resin and improved on-grid immunogold detection of vicilin, a pea seed storage protein.

Abstract

LR White resin combined with post-embedding immunogold labelling was used to localize the storage protein vicilin within developing pea seed cotyledons by electron microscopy. Fine structural preservation is comparable to that obtained with Spurr's resin, and antibody labelling is improved. More gold binds to protein bodies, to rough endoplasmic reticulum and to Golgi vesicles, and in addition, vicilin was detected within Golgi cisternae, a site not previously observed.

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Year: 1984 PMID： 6509563 DOI： 10.1016/0309-1651(84)90072-9

Source DB: PubMed Journal: Cell Biol Int Rep ISSN： 0309-1651

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Cited

23 in total

1. Correct targeting of the bean storage protein phaseolin in the seeds of transformed tobacco.

Authors: J S Greenwood; M J Chrispeels
Journal: Plant Physiol Date: 1985-09 Impact factor: 8.340

2. Bark and Leaf Lectins of Sophora japonica Are Sequestered in Protein-Storage Vacuoles.

Authors: E M Herman; C N Hankins; L M Shannon
Journal: Plant Physiol Date: 1988-04 Impact factor: 8.340

3. The sequence of a pea vicilin gene and its expression in transgenic tobacco plants.

Authors: T J Higgins; E J Newbigin; D Spencer; D J Llewellyn; S Craig
Journal: Plant Mol Biol Date: 1988-09 Impact factor: 4.076

4. Intracellular localisation of phytochrome in oat coleoptiles by electron microscopy.

Authors: V Speth; V Otto; E Schäfer
Journal: Planta Date: 1986-09 Impact factor: 4.116

5. Localization of Bacteria and Hemoglobin in Root Nodules of Parasponia andersonii Containing Both Bradyrhizobium Strains and Rhizobium leguminosarum biovar trifolii.

Authors: Michael J Trinick; David J Goodchild; Celia Miller
Journal: Appl Environ Microbiol Date: 1989-08 Impact factor: 4.792

6. A new polychrome stain and simultaneous methods of histological, histochemical and immunohistochemical stainings performed on semithin sections of Bioacryl-embedded human tissues.

Authors: C Scala; P Preda; G Cenacchi; G N Martinelli; G C Manara; G Pasquinelli
Journal: Histochem J Date: 1993-09

LR white resin and improved on-grid immunogold detection of vicilin, a pea seed storage protein.

1. Correct targeting of the bean storage protein phaseolin in the seeds of transformed tobacco.

2. Bark and Leaf Lectins of Sophora japonica Are Sequestered in Protein-Storage Vacuoles.

3. The sequence of a pea vicilin gene and its expression in transgenic tobacco plants.

4. Intracellular localisation of phytochrome in oat coleoptiles by electron microscopy.

5. Localization of Bacteria and Hemoglobin in Root Nodules of Parasponia andersonii Containing Both Bradyrhizobium Strains and Rhizobium leguminosarum biovar trifolii.

6. A new polychrome stain and simultaneous methods of histological, histochemical and immunohistochemical stainings performed on semithin sections of Bioacryl-embedded human tissues.

7. Fertilization-independent seed development in Arabidopsis thaliana.

8. Immunolocalization of avenin and globulin storage proteins in developing endosperm of Avena sativa L.

9. Evidence of Oxidative Phosphorylation in Zebrafish Photoreceptor Outer Segments at Different Larval Stages.

10. Immunocytochemical localization of actin in dendritic spines of the cerebral cortex using colloidal gold as a probe.