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Literature DB >> 6508760

A modified perifused incubation system for isolated fat-cells. Investigation of intermediary metabolism by perifusion.

Abstract

Perifused fat-cells showed similar values for acylglycerol glycerol synthesis from glucose with insulin and for the effects of added palmitate to those in normal incubations and those reported in the literature. Fatty acid synthesis was lower in perifused cells compared with normal incubations, and there was a net release of fatty acids only with the perifused fat-cells. Hence fluxes of metabolites were different in the two incubation systems, and the perifusion system enables the investigation of the flux of metabolites under conditions which may more closely resemble those in vivo.

Entities: Chemical Gene

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Year: 1984 PMID： 6508760 PMCID： PMC1144418 DOI： 10.1042/bj2240235

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

26 in total

1. Feeding and breeding of laboratory animals; a complete cubed diet for mice and rats.

Authors: H M BRUCE; A S PARKES
Journal: J Hyg (Lond) Date: 1949-06

2. Spectrum of fatty acids synthesized in situ and metabolic heterogeneity of free fatty acids and glycerides within isolated rat adipocytes.

Authors: J Winand; J Furnelle; C Wodon; J Christophe
Journal: Biochim Biophys Acta Date: 1971-07-13

3. Effect of ACTH on FFA release and diglyceride content in perfused rat adipose tissue.

Authors: R O Scow; F A Stricker; T Y Pick; T R Clary
Journal: Ann N Y Acad Sci Date: 1965-10-08 Impact factor: 5.691

4. The regulation of glyceride synthesis from pyruvate in isolated fat cells. The effects of palmitate and alteration of dietary status.

Authors: E D Saggerson; G Tomassi
Journal: Eur J Biochem Date: 1971-11-11

10. Measurement of concentrations of metabolites in adipose tissue and effects of insulin, alloxan-diabetes and adrenaline.

Authors: R M Denton; R E Yorke; P J Randle
Journal: Biochem J Date: 1966-08 Impact factor: 3.857

1 in total

1. The response of adipocyte glucose metabolism and fatty acid release to adenosine deaminase, insulin and perifusion. Investigation of intermediary metabolism by perifusion.

Authors: R D Harper
Journal: Biochem J Date: 1988-05-01 Impact factor: 3.857

1 in total

A modified perifused incubation system for isolated fat-cells. Investigation of intermediary metabolism by perifusion.

1. Feeding and breeding of laboratory animals; a complete cubed diet for mice and rats.

2. Spectrum of fatty acids synthesized in situ and metabolic heterogeneity of free fatty acids and glycerides within isolated rat adipocytes.

3. Effect of ACTH on FFA release and diglyceride content in perfused rat adipose tissue.

4. The regulation of glyceride synthesis from pyruvate in isolated fat cells. The effects of palmitate and alteration of dietary status.

5. Intracellular accumulation of free fatty acids in isolated white adipose cells.

6. Effect of cell size on the metabolism of isolated fat cells.

7. Colorimetric micro-determination of free fatty acids in plasma.

8. The regulation of glyceride synthesis in isolated white-fat cells. The effects of palmitate and lipolytic agents.

9. The regulation of triglyceride synthesis and fatty acid synthesis in rat epididymal adipose tissue.

10. Measurement of concentrations of metabolites in adipose tissue and effects of insulin, alloxan-diabetes and adrenaline.

1. The response of adipocyte glucose metabolism and fatty acid release to adenosine deaminase, insulin and perifusion. Investigation of intermediary metabolism by perifusion.