Literature DB >> 6508749

Isolation of rat mammary epithelial cells for polyamine analysis.

R Minocha, H J Thompson, L D Meeker, E J Herbst.   

Abstract

Mammary epithelial cells were isolated from either abdominal-inguinal glands or mammary tumours of rats, after enzymic digestion of the tissues, and were analysed for polyamine content. Optimum conditions were developed for the isolation of cells in sufficient yield for the analysis of polyamines from 1 g of mammary gland or 0.5 g of tumour tissue. Complete recoveries of the polyamines in the tissues were achieved in the isolated epithelial cells.

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Year:  1984        PMID： 6508749      PMCID： PMC1144382          DOI： 10.1042/bj2230929

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  19 in total

1.  Techniques for studying development of normal mammary epithelial cells in organ culture.

Authors:  R J Topper; T Oka; B K Vonderhaar
Journal:  Methods Enzymol       Date:  1975       Impact factor: 1.600

2.  A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.

Authors:  K BURTON
Journal:  Biochem J       Date:  1956-02       Impact factor: 3.857

3.  Putrescine and related compounds as growth factors for Hemophilus parainfluenzae 7991.

Authors:  E J HERBST; E E SNELL
Journal:  J Biol Chem       Date:  1949-11       Impact factor: 5.157

Review 4.  Polyamines in rapid growth and cancer.

Authors:  J Jänne; H Pösö; A Raina
Journal:  Biochim Biophys Acta       Date:  1978-04-06

5.  Preparation of fat cell-"free" rat mammary gland.

Authors:  R C Moon; D H Janss; S Young
Journal:  J Histochem Cytochem       Date:  1969-03       Impact factor: 2.479

6.  A technique for isolating pure, viable parenchymal cells and their nuclei from rat mammary gland.

Authors:  L C Minasian; A G Jabara
Journal:  Anal Biochem       Date:  1974-04       Impact factor: 3.365

7.  [Microdetermination of spermine and spermidine as 1-dimethylaminonaphthalene-5-sulfonic acid derivtives].

Authors:  N Seiler; M Wiechmann
Journal:  Hoppe Seylers Z Physiol Chem       Date:  1967-10

8.  Regulation of the activity of ornithine decarboxylase and S-adenosylmethionine decarboxylase in mammary gland and liver of lactating rats. Effects of starvation, prolactin and insulin deficiency.

Authors:  M E Brosnan; V Ilic; D H Williamson
Journal:  Biochem J       Date:  1982-03-15       Impact factor: 3.857

Review 9.  Polyamine metabolism and function.

Authors:  A E Pegg; P P McCann
Journal:  Am J Physiol       Date:  1982-11

10.  Lifetime dose-response relationships for mammary tumor induction by a single administration of N-methyl-N-nitrosourea.

Authors:  D L McCormick; C B Adamowski; A Fiks; R C Moon
Journal:  Cancer Res       Date:  1981-05       Impact factor: 12.701
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