Literature DB >> 6508737

Purification and properties of a beta-1,6-glucanase from Penicillium brefeldianum.

G P Schep, M G Shepherd, P A Sullivan.   

Abstract

An inducible endo-beta-1,6-glucanase was purified from Penicillium brefeldianum by DEAE-cellulose, Bio-Gel P-150 and high-pressure liquid chromatography. The final preparation was essentially free from beta-1,3-glucanase and beta-glucosidase activities. Sodium dodecyl sulphate/polyacrylamide-gel electrophoresis revealed one protein band with an Mr of 44000. The Vmax. and Km values were calculated to be 624 units (mumol/min)/mg and 2.78 mg/ml respectively. The glucanase had lytic activity against mycelial cells of the yeast Candida albicans. The yield of purified beta-1,6-glucanase from 100 mg dry weight of freeze-dried culture filtrate varied from 60 to 180 units.

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Year:  1984        PMID： 6508737      PMCID： PMC1144354          DOI： 10.1042/bj2230707

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  15 in total

1.  Beta-d-1, 6-Glucanases in fungi.

Authors:  E T REESE; F W PARRISH; M MANDELS
Journal:  Can J Microbiol       Date:  1962-06       Impact factor: 2.419

2.  An endo-(1 lead to 6) -beta-D-glucanase from Mucor hiemalis.

Authors:  T Miyazaki; N Oikawa
Journal:  Carbohydr Res       Date:  1976-06       Impact factor: 2.104

3.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

4.  Lysis of yeast cell walls. Lytic beta-(1 leads to 6)-glucanase from Bacillus circulans WL-12.

Authors:  F M Rombouts; H J Phaff
Journal:  Eur J Biochem       Date:  1976-03-16

5.  Colorimetric and fluorometric carbohydrate determination with p-hydroxybenzoic acid hydrazide.

Authors:  M Lever
Journal:  Biochem Med       Date:  1973-04

6.  An improved method for enzymic determination of glucose in the presence of maltose.

Authors:  J B Lloyd; W J Whelan
Journal:  Anal Biochem       Date:  1969-09       Impact factor: 3.365

7.  Production and catabolite repression of Penicillium italicum beta-glucanases.

Authors:  T Santos; J R Villanueva; C Nombela
Journal:  J Bacteriol       Date:  1977-01       Impact factor: 3.490

8.  Characterization and synthesis regulation of Penicillium italicum 1,6-beta-glucanase.

Authors:  T Santos; C Nombela; J R Villanueva; G Larriba
Journal:  Arch Microbiol       Date:  1979-06       Impact factor: 2.552

9.  SDS microslab linear gradient polyacrylamide gel electrophoresis.

Authors:  P T Matsudaira; D R Burgess
Journal:  Anal Biochem       Date:  1978-07-01       Impact factor: 3.365

10.  Lysis of yeast cell walls: glucanases from Bacillus circulans WL-12.

Authors:  G H Fleet; H J Phaff
Journal:  J Bacteriol       Date:  1974-07       Impact factor: 3.490
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  8 in total

1.  Tvbgn3, a beta-1,6-glucanase from the biocontrol fungus Trichoderma virens, is involved in mycoparasitism and control of Pythium ultimum.

Authors:  Slavica Djonović; Maria J Pozo; Charles M Kenerley
Journal:  Appl Environ Microbiol       Date:  2006-09-22       Impact factor: 4.792

2.  Endophytic fungal beta-1,6-glucanase expression in the infected host grass.

Authors:  Melinda Moy; Huaijun Michael Li; Ray Sullivan; James F White; Faith C Belanger
Journal:  Plant Physiol       Date:  2002-11       Impact factor: 8.340

3.  Candida albicans mycelial wall structure: supramolecular complexes released by zymolyase, chitinase and beta-mercaptoethanol.

Authors:  A Marcilla; M V Elorza; S Mormeneo; H Rico; R Sentandreu
Journal:  Arch Microbiol       Date:  1991       Impact factor: 2.552

4.  Purification and characterization of an extracellular (1 --> 6)-beta-glucanase from the filamentous fungus Acremonium persicinum.

Authors:  S M Pitson; R J Seviour; B M McDougall; B A Stone; M Sadek
Journal:  Biochem J       Date:  1996-06-15       Impact factor: 3.857

5.  Two exo-beta-D-glucosaminidases/exochitosanases from actinomycetes define a new subfamily within family 2 of glycoside hydrolases.

Authors:  Nathalie Côté; Alain Fleury; Emilie Dumont-Blanchette; Tamo Fukamizo; Masaru Mitsutomi; Ryszard Brzezinski
Journal:  Biochem J       Date:  2006-03-15       Impact factor: 3.857

6.  The purification and properties of yeast proteinase B from Candida albicans.

Authors:  P C Farley; M G Shepherd; P A Sullivan
Journal:  Biochem J       Date:  1986-05-15       Impact factor: 3.857

7.  Diversity of family GH46 chitosanases in Kitasatospora setae KM-6054.

Authors:  Mina Zitouni; Pascal Viens; Mariana G Ghinet; Ryszard Brzezinski
Journal:  Appl Microbiol Biotechnol       Date:  2017-09-18       Impact factor: 4.813

8.  Biochemical Characterization of Recombinant Thermostable Cohnella sp. A01 β-Glucanase

Authors:  Meysam Rezaie; Saeed Aminzadeh; Farid Heidari; Masoud Mashhadi Akbar Boojar; Ali Asghar Karkhane
Journal:  Iran Biomed J       Date:  2018-01-13
  8 in total

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