Literature DB >> 6500063

In vitro enzymatic methylation of DNA substituted by N-2-aminofluorene.

A Pfohl-Leszkowicz, R P Fuchs, G Dirheimer.   

Abstract

Both the initial velocity and the overall methylation of DNA substituted by aminofluorene, by a rat liver DNA(cytosine-5-)-methyltransferase, are increased as compared to native DNA. The Km and Vmax of the modified DNA for the enzyme increase as a function of the extent of modification. The carcinogen may induce a secondary structure favouring the 'walking' of the enzyme along the DNA. The hypermethylation caused by this carcinogen could have a significance in gene activity, cellular differentiation and cancer induction.

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Year:  1984        PMID： 6500063     DOI： 10.1016/0014-5793(84)81240-5

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  2 in total

1.  Chemical carcinogen-induced decreases in genomic 5-methyldeoxycytidine content of normal human bronchial epithelial cells.

Authors:  V L Wilson; R A Smith; J Longoria; M A Liotta; C M Harper; C C Harris
Journal:  Proc Natl Acad Sci U S A       Date:  1987-05       Impact factor: 11.205

2.  Activation of a nonexpressed hypoxanthine phosphoribosyltransferase allele in mutant H23 HeLa cells by agents that inhibit DNA methylation.

Authors:  R Ivarie; J A Morris
Journal:  Mol Cell Biol       Date:  1986-01       Impact factor: 4.272
  2 in total

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