Literature DB >> 6499138

Measurement of cytosolic free calcium concentration in isolated rat ventricular myocytes with quin 2.

S S Sheu, V K Sharma, S P Banerjee.   

Abstract

Cytosolic free calcium concentration was determined in isolated ventricular myocytes from adult rats with the calcium-sensitive indicator, quin 2. The fluorescence signal from resting cells indicated that cytosolic free calcium concentration was 181 +/- 18 nM (mean +/- SEM, n = 18). Inhibition of the sodium-potassium pump with strophanthidin (0.1 mM) resulted in an increase of cytosolic free calcium concentration from 186 +/- 17 to 736 +/- 129 nM (n = 6). The results indicate that it is possible to measure cytosolic free calcium concentration in cardiac muscle cells that have been isolated enzymatically. Moreover, they confirm the observation that inhibition of the sodium-potassium pump increases cytosolic free calcium concentration, presumably via the sodium-calcium exchange mechanism.

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Year:  1984        PMID： 6499138     DOI： 10.1161/01.res.55.6.830

Source DB:  PubMed          Journal:  Circ Res        ISSN： 0009-7330            Impact factor:   17.367


  21 in total

1.  Mechanism of extracellular ATP-induced increase of cytosolic Ca2+ concentration in isolated rat ventricular myocytes.

Authors:  A Christie; V K Sharma; S S Sheu
Journal:  J Physiol       Date:  1992-01       Impact factor: 5.182

2.  Thermodynamic linkage between calmodulin domains binding calcium and contiguous sites in the C-terminal tail of Ca(V)1.2.

Authors:  T Idil Apak Evans; Johannes W Hell; Madeline A Shea
Journal:  Biophys Chem       Date:  2011-06-24       Impact factor: 2.352

3.  Sodium/calcium exchange and intracellular calcium buffering in ferret myocardium: an ion-sensitive micro-electrode study.

Authors:  R A Chapman
Journal:  J Physiol       Date:  1986-04       Impact factor: 5.182

4.  Altered adenosine triphosphatase activities in pigs with naturally occurring hypertrophic cardiomyopathy.

Authors:  K S Dai; C S Liang; Y T Ch'iu; P C Yang; I C Cheng
Journal:  Vet Res Commun       Date:  1995       Impact factor: 2.459

5.  Regulation of mitochondrial fission by intracellular Ca2+ in rat ventricular myocytes.

Authors:  Jennifer Hom; Tianzheng Yu; Yisang Yoon; George Porter; Shey-Shing Sheu
Journal:  Biochim Biophys Acta       Date:  2010-03-27

6.  Relationship between calcium loading and impaired energy metabolism during Na+, K+ pump inhibition and metabolic inhibition in cultured neonatal rat cardiac myocytes.

Authors:  A C Morris; H K Hagler; J T Willerson; L M Buja
Journal:  J Clin Invest       Date:  1989-06       Impact factor: 14.808

Review 7.  Sodium/calcium exchange in ventricular muscle.

Authors:  J A McGuigan; L A Blatter
Journal:  Experientia       Date:  1987-12-01

8.  Mitochondrial fusion dynamics is robust in the heart and depends on calcium oscillations and contractile activity.

Authors:  Verónica Eisner; Ryan R Cupo; Erhe Gao; György Csordás; William S Slovinsky; Melanie Paillard; Lan Cheng; Jessica Ibetti; S R Wayne Chen; J Kurt Chuprun; Jan B Hoek; Walter J Koch; György Hajnóczky
Journal:  Proc Natl Acad Sci U S A       Date:  2017-01-17       Impact factor: 11.205

9.  Effect of common agonists on cytoplasmic ionized calcium concentration in platelets. Measurement with 2-methyl-6-methoxy 8-nitroquinoline (quin2) and aequorin.

Authors:  J A Ware; P C Johnson; M Smith; E W Salzman
Journal:  J Clin Invest       Date:  1986-03       Impact factor: 14.808

10.  Cytosolic calcium transients from the beating mammalian heart.

Authors:  H C Lee; N Smith; R Mohabir; W T Clusin
Journal:  Proc Natl Acad Sci U S A       Date:  1987-11       Impact factor: 11.205
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