Literature DB >> 6497861

An improved procedure for the isolation of intact chloroplasts of high photosynthetic capacity.

Z G Cerović, M Plesnicar.   

Abstract

An improved procedure for the mechanical isolation of chloroplasts of high degree of intactness (90-95%) and photosynthetic capacity (25-50 mmol of O2/s per mol of chlorophyll) is described. The combination of pea plants (Pisum sativum L.) as starting material and the high reproducibility of the procedure readily and cheaply yields reliable intact chloroplasts for photosynthetic studies.

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Mesh：

Year:  1984        PMID： 6497861      PMCID： PMC1144329          DOI： 10.1042/bj2230543

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  4 in total

1.  An improved method for isolating chloroplasts retaining their outer membranes.

Authors:  H Y Nakatani; J Barber
Journal:  Biochim Biophys Acta       Date:  1977-09-14

2.  Nomenclature for isolated chloroplasts.

Authors:  D O Hall
Journal:  Nat New Biol       Date:  1972-01-26

3.  Hydrogen ion buffers for biological research.

Authors:  N E Good; G D Winget; W Winter; T N Connolly; S Izawa; R M Singh
Journal:  Biochemistry       Date:  1966-02       Impact factor: 3.162

4.  Slow Passive Diffusion of Orthophosphate between Intact Isolated Chloroplasts and Suspending Medium.

Authors:  G Mourioux; R Douce
Journal:  Plant Physiol       Date:  1981-03       Impact factor: 8.340
  4 in total
  20 in total

1.  Light- and pH-dependent structural changes in the PsbS subunit of photosystem II.

Authors:  Elisabetta Bergantino; Anna Segalla; Alessia Brunetta; Enrico Teardo; Fernanda Rigoni; Giorgio Mario Giacometti; Ildikò Szabò
Journal:  Proc Natl Acad Sci U S A       Date:  2003-12-01       Impact factor: 11.205

2.  Compared thermoluminescence characteristics of pea thylakoids studied in vitro and in situ (in leaves). The effect of photoinhibitory treatments.

Authors:  J Farineau
Journal:  Photosynth Res       Date:  1993-04       Impact factor: 3.573

3.  The role of transmembrane electrochemical potential and phosphorylation of PS II proteins in temperature induced light emission from ATP-treated lettuce thylakoids.

Authors:  J Farineau
Journal:  Photosynth Res       Date:  1996-03       Impact factor: 3.573

4.  Induction of oscillations in chlorophyll fluorescence by re-illumination of intact isolated pea chloroplasts.

Authors:  S Veljović-Jovanović; Z G Cerović
Journal:  Planta       Date:  1991-10       Impact factor: 4.116

5.  Regulation of plant Fatty Acid biosynthesis : analysis of acyl-coenzyme a and acyl-acyl carrier protein substrate pools in spinach and pea chloroplasts.

Authors:  D Post-Beittenmiller; G Roughan; J B Ohlrogge
Journal:  Plant Physiol       Date:  1992-10       Impact factor: 8.340

6.  Plant dihydroxyacetone phosphate reductases : purification, characterization, and localization.

Authors:  T Kirsch; D W Gerber; R U Byerrum; N E Tolbert
Journal:  Plant Physiol       Date:  1992-09       Impact factor: 8.340

7.  Characterisation of the effects of Antimycin A upon high energy state quenching of chlorophyll fluorescence (qE) in spinach and pea chloroplasts.

Authors:  K Oxborough; P Horton
Journal:  Photosynth Res       Date:  1987-01       Impact factor: 3.573

8.  Association of glycolate oxidation with photosynthetic electron transport in plant and algal chloroplasts.

Authors:  A Goyal; N E Tolbert
Journal:  Proc Natl Acad Sci U S A       Date:  1996-04-16       Impact factor: 11.205

9.  The accumulation of a cold-regulated chloroplastic protein is light-dependent.

Authors:  C Crosatti; C Soncini; A M Stanca; L Cattivelli
Journal:  Planta       Date:  1995       Impact factor: 4.116

10.  Evidence That Isolated Chloroplasts Contain an Integrated Lipid-Synthesizing Assembly That Channels Acetate into Long-Chain Fatty Acids.

Authors:  P. G. Roughan; J. B. Ohlrogge
Journal:  Plant Physiol       Date:  1996-04       Impact factor: 8.340
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