Literature DB >> 6496946

An enzymatic fluorimetric assay for myo-inositol.

L C MacGregor, F M Matschinsky.   

Abstract

An enzymatic assay for myo-inositol (MI) is described. The method is based on the oxidation of MI by NAD+-dependent myo-inositol dehydrogenase, coupled to reoxidation of NADH with oxalacetate and malate dehydrogenase. The resultant malate is measured fluorimetrically. Several variations of the assay are described for measuring MI in serum and in tissues in amounts ranging from 0.2 pmol to 8 nmol. Highest sensitivity is achieved by applying an oil-well technique for handling small droplets, and by using the principle of enzymatic cycling. The potential of the technique is illustrated by MI measurements in several tissues of normal and diabetic rats and Chinese hamsters.

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Year:  1984        PMID： 6496946     DOI： 10.1016/0003-2697(84)90058-7

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  15 in total

1.  Determination of transport stoichiometry for two cation-coupled myo-inositol cotransporters: SMIT2 and HMIT.

Authors:  Francis Bourgeois; Michael J Coady; Jean-Yves Lapointe
Journal:  J Physiol       Date:  2004-12-21       Impact factor: 5.182

2.  The diabetic rat kidney mediates inosituria and selective urinary partitioning of D-chiro-inositol.

Authors:  Hao-Han Chang; Bernard Choong; Anthony R J Phillips; Kerry M Loomes
Journal:  Exp Biol Med (Maywood)       Date:  2014-07-24

3.  Osmoregulatory changes in myo-inositol transport by renal cells.

Authors:  T Nakanishi; R J Turner; M B Burg
Journal:  Proc Natl Acad Sci U S A       Date:  1989-08       Impact factor: 11.205

4.  Product-precursor relationships amongst inositol polyphosphates. Incorporation of [32P]Pi into myo-inositol 1,3,4,6-tetrakisphosphate, myo-inositol 1,3,4,5-tetrakisphosphate, myo-inositol 3,4,5,6-tetrakisphosphate and myo-inositol 1,3,4,5,6-pentakisphosphate in intact avian erythrocytes.

Authors:  L R Stephens; C P Downes
Journal:  Biochem J       Date:  1990-01-15       Impact factor: 3.857

5.  Measurement of picomole amounts of any inositol phosphate isomer separable by h.p.l.c. by means of a bioluminescence assay.

Authors:  S A Prestwich; T B Bolton
Journal:  Biochem J       Date:  1991-03-15       Impact factor: 3.857

6.  Active transport of myo-inositol in rat pancreatic islets.

Authors:  T J Biden; C B Wollheim
Journal:  Biochem J       Date:  1986-06-15       Impact factor: 3.857

7.  Quantitative determination of free glycerol and myo-inositol from plasma and tissue by high-performance liquid chromatography.

Authors:  Ryan A Frieler; Dane J Mitteness; Mikhail Y Golovko; Heidi M Gienger; Thad A Rosenberger
Journal:  J Chromatogr B Analyt Technol Biomed Life Sci       Date:  2009-09-19       Impact factor: 3.205

8.  Chronic exposure to high glucose decreases myo-inositol in cultured cerebral microvascular pericytes but not in endothelium.

Authors:  I Sussman; M P Carson; V Schultz; X P Wu; A L McCall; N B Ruderman; K Tornheim
Journal:  Diabetologia       Date:  1988-10       Impact factor: 10.122

9.  Radio-label and mass determinations of inositol 1,3,4,5-tetrakisphosphate formation in rat cerebral cortical slices: differential effects of myo-inositol.

Authors:  P Kurian; N Narang; L J Chandler; F T Crews
Journal:  Neurochem Res       Date:  1993-05       Impact factor: 3.996

10.  Evidence for attenuation of myo-inositol uptake, phosphoinositide turnover and inositol phosphate production in aortic vasculature of rats during pregnancy.

Authors:  K P Conrad; S A Barrera; P A Friedman; V M Schmidt
Journal:  J Clin Invest       Date:  1991-05       Impact factor: 14.808
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