Literature DB >> 6496932

Preparation of chloroplast DNA from pea plastids isolated in a medium of high ionic strength.

G Bookjans, B M Stummann, K W Henningsen.   

Abstract

A simple, rapid, and inexpensive method for the preparation and purification of chloroplast DNA (cpDNA) from pea has been developed. The crucial step is the isolation of chloroplasts in a medium of high ionic strength (I congruent equal to 1.40 M). CpDNA from pea prepared according to this method has successfully been used for restriction enzyme mapping, Southern transfers, and cloning.

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Year:  1984        PMID： 6496932     DOI： 10.1016/0003-2697(84)90452-4

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  41 in total

1.  Targeted inactivation of the plastid ndhB gene in tobacco results in an enhanced sensitivity of photosynthesis to moderate stomatal closure.

Authors:  E M Horváth; S O Peter; T Joët; D Rumeau; L Cournac; G V Horváth; T A Kavanagh; C Schäfer; G Peltier; P Medgyesy
Journal:  Plant Physiol       Date:  2000-08       Impact factor: 8.340

2.  C4 Gene Expression in Photosynthetic and Nonphotosynthetic Leaf Regions of Amaranthus tricolor.

Authors:  D. McCormac; J. J. Boinski; V. C. Ramsperger; J. O. Berry
Journal:  Plant Physiol       Date:  1997-07       Impact factor: 8.340

3.  Localized hypermutation and associated gene losses in legume chloroplast genomes.

Authors:  Alan M Magee; Sue Aspinall; Danny W Rice; Brian P Cusack; Marie Sémon; Antoinette S Perry; Sasa Stefanović; Dan Milbourne; Susanne Barth; Jeffrey D Palmer; John C Gray; Tony A Kavanagh; Kenneth H Wolfe
Journal:  Genome Res       Date:  2010-10-26       Impact factor: 9.043

4.  T7 RNA polymerase-directed expression of an antibody fragment transgene in plastids causes a semi-lethal pale-green seedling phenotype.

Authors:  Alan M Magee; Seamus Coyne; David Murphy; Eva M Horvath; Peter Medgyesy; Tony A Kavanagh
Journal:  Transgenic Res       Date:  2004-08       Impact factor: 2.788

5.  Nucleotide sequence of the chloroplast 16S rRNA gene from pea (Pisum sativum L.).

Authors:  H Cerutti; A T Jagendorf
Journal:  Plant Mol Biol       Date:  1991-07       Impact factor: 4.076

6.  Structure of a 3.2 kb region of pea chloroplast DNA containing the gene for the 44 kD photosystem II polypeptide.

Authors:  G Bookjans; B M Stummann; O F Rasmussen; K W Henningsen
Journal:  Plant Mol Biol       Date:  1986-09       Impact factor: 4.076

7.  Sequence of two genes in pea chloroplast DNA coding for 84 and 82 kD polypeptides of the photosystem I complex.

Authors:  J Lehmbeck; O F Rasmussen; G B Bookjans; B R Jepsen; B M Stummann; K W Henningsen
Journal:  Plant Mol Biol       Date:  1986-01       Impact factor: 4.076

8.  A model for the evolution of the Vicia faba chloroplast genome.

Authors:  K Ko; A G Orfanides; N A Straus
Journal:  Theor Appl Genet       Date:  1987-05       Impact factor: 5.699

9.  RFLP analysis of mitochondrial DNA from cytoplasmic male-sterile lines of pearl millet.

Authors:  R Rajeshwari; S Sivaramakrishnan; R L Smith; N C Subrahmanyam
Journal:  Theor Appl Genet       Date:  1994-06       Impact factor: 5.699

10.  Outcrossing and hybridization in wild and cultivated foxtail millets: consequences for the release of transgenic crops.

Authors:  I Till-Bottraud; X Reboud; P Brabant; M Lefranc; B Rherissi; F Vedel; H Darmency
Journal:  Theor Appl Genet       Date:  1992-05       Impact factor: 5.699
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