Literature DB >> 6496928

A competitive displacement assay to detect saxitoxin and tetrodotoxin.

S R Davio, P A Fontelo.   

Abstract

An assay is described which detects saxitoxin (STX) and tetrodotoxin (TTX) by their competitive displacement of [3H]saxitoxin from its receptor in rat brain membranes. The assay has a sensitivity of 0.15 ng STX/ml and 0.8 ng TTX/ml for buffer samples. The assay was also applied to detection of these toxins in unextracted human plasma and found to have a sensitivity of 0.5 ng STX/ml and 0.6 ng TTX/ml. The competitive displacement assay appears to be the most sensitive procedure yet for detection of STX and TTX.

Entities:  

Mesh：

Substances：

Year:  1984        PMID： 6496928     DOI： 10.1016/0003-2697(84)90446-9

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  8 in total

1.  Lack of specific saxitoxin binding to rat mast cells.

Authors:  M R Vieytes; M C Louzao; M J Bujan; A Alfonso; A Cabado; L F de la Cruz; L M Botana
Journal:  Agents Actions       Date:  1991-05

2.  Radioimmunoassay of paralytic shellfish toxins in clams and mussels.

Authors:  G C Yang; S J Imagire; P Yasaei; E P Ragelis; D L Park; S W Page; R E Carlson; P E Guire
Journal:  Bull Environ Contam Toxicol       Date:  1987-08       Impact factor: 2.151

3.  Emerging Lyngbya wollei toxins: A new high resolution mass spectrometry method to elucidate a potential environmental threat.

Authors:  Meagan L Smith; Danielle C Westerman; Samuel P Putnam; Susan D Richardson; John L Ferry
Journal:  Harmful Algae       Date:  2019-11-20       Impact factor: 4.273

4.  Protection against nerve toxicity by monoclonal antibodies to the sodium channel blocker tetrodotoxin.

Authors:  R I Huot; D L Armstrong; T C Chanh
Journal:  J Clin Invest       Date:  1989-06       Impact factor: 14.808

5.  Fluidic force discrimination assays: a new technology for tetrodotoxin detection.

Authors:  Betsy Jean Yakes; Stacey M Etheridge; Shawn P Mulvaney; Cy R Tamanaha
Journal:  Mar Drugs       Date:  2010-03-10       Impact factor: 5.118

Review 6.  Potential Threats Posed by Tetrodotoxins in UK Waters: Examination of Detection Methodology Used in Their Control.

Authors:  Andrew D Turner; Cowan Higgins; Wendy Higman; James Hungerford
Journal:  Mar Drugs       Date:  2015-12-11       Impact factor: 5.118

Review 7.  An overview on the marine neurotoxin, saxitoxin: genetics, molecular targets, methods of detection and ecological functions.

Authors:  Kathleen D Cusick; Gary S Sayler
Journal:  Mar Drugs       Date:  2013-03-27       Impact factor: 5.118

Review 8.  Guanidinium Toxins and Their Interactions with Voltage-Gated Sodium Ion Channels.

Authors:  Lorena M Durán-Riveroll; Allan D Cembella
Journal:  Mar Drugs       Date:  2017-10-13       Impact factor: 5.118
  8 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.