Literature DB >> 6496922

Determination of ketone body turnover in vivo with stable isotopes, utilizing gas chromatography/mass spectrometry.

J M Miles, W F Schwenk, K L McClean, M W Haymond.   

Abstract

JH imple and reliable method for the determination of ketone body turnover in vivo using a primed, continuous infusion of [3,4-13C2]acetoacetate is described. Mole percent enrichment of beta-[13C2]hydroxybutyrate and [13C2]acetoacetate is determined by gas chromatography/mass spectrometry using electron-impact ionization and selected ion monitoring. Ketone body flux data are provided from preliminary dog experiments. The method is readily applicable to the study of ketone body metabolism in both laboratory animals and humans.

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Year:  1984        PMID： 6496922     DOI： 10.1016/0003-2697(84)90432-9

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  3 in total

1.  Ketone body kinetics in vivo using simultaneous administration of acetoacetate and 3-hydroxybutyrate labelled with stable isotopes.

Authors:  A Avogaro; R Nosadini; D M Bier; C Cobelli; G Toffolo; A Doria; A Valerio; H Christopherson
Journal:  Acta Diabetol Lat       Date:  1990 Jan-Mar

2.  Determination of human ketone body kinetics using stable-isotope labelled tracers.

Authors:  M Beylot; B Beaufrère; S Normand; J P Riou; R Cohen; R Mornex
Journal:  Diabetologia       Date:  1986-02       Impact factor: 10.122

3.  Validation of a Gas Chromatography-Mass Spectrometry Method for the Measurement of the Redox State Metabolic Ratios Lactate/Pyruvate and β-Hydroxybutyrate/Acetoacetate in Biological Samples.

Authors:  Robin Wijngaard; Meritxell Perramón; Marina Parra-Robert; Susana Hidalgo; Gina Butrico; Manuel Morales-Ruiz; Muling Zeng; Eudald Casals; Wladimiro Jiménez; Guillermo Fernández-Varo; Gerald I Shulman; Gary W Cline; Gregori Casals
Journal:  Int J Mol Sci       Date:  2021-04-30       Impact factor: 5.923
  3 in total

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