Literature DB >> 6491300

Cobra venom factor: improved method for purification and biochemical characterization.

C W Vogel, H J Müller-Eberhard.   

Abstract

A method to purify cobra venom factor (CVF) from cobra venom by sequential column chromatography is described which yields a product virtually free of phospholipase A2, a common contaminant of CVF preparations. The separation of phospholipase A2 from CVF was achieved by chromatography on Cibacron blue-agarose, a resin that tightly binds cobra venom phospholipase A2. A rapid and simple hemolytic assay for the qualitative and quantitative determination of CVF based on its ability to induce bystander lysis of erythrocytes has been devised. CVF was isolated from the venom of the Naja naja kaouthia subspecies and some of its biochemical properties and physicochemical parameters were delineated.

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Year:  1984        PMID： 6491300     DOI： 10.1016/0022-1759(84)90045-0

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  31 in total

1.  A nonhuman primate model for the selective elimination of CD8+ lymphocytes using a mouse-human chimeric monoclonal antibody.

Authors:  J E Schmitz; M A Simon; M J Kuroda; M A Lifton; M W Ollert; C W Vogel; P Racz; K Tenner-Racz; B J Scallon; M Dalesandro; J Ghrayeb; E P Rieber; V G Sasseville; K A Reimann
Journal:  Am J Pathol       Date:  1999-06       Impact factor: 4.307

2.  Isolation and characterization of the complement-inhibiting protein CD59 antigen from platelet membranes.

Authors:  B P Morgan
Journal:  Biochem J       Date:  1992-03-01       Impact factor: 3.857

3.  Characterization of the membrane attack complex inhibitory protein CD59 antigen on human amniotic cells and in amniotic fluid.

Authors:  I A Rooney; B P Morgan
Journal:  Immunology       Date:  1992-08       Impact factor: 7.397

4.  Effect of N-methylation on the modulation by synthetic peptides of the activity of the complement-factor-B-derived serine proteinase CVFBb.

Authors:  A Berkovich; M C O'Keefe; P Hensley; L Caporale
Journal:  Biochem J       Date:  1990-09-01       Impact factor: 3.857

5.  Insights into complement convertase formation based on the structure of the factor B-cobra venom factor complex.

Authors:  Bert J C Janssen; Lucio Gomes; Roman I Koning; Dmitri I Svergun; Abraham J Koster; David C Fritzinger; Carl-Wilhelm Vogel; Piet Gros
Journal:  EMBO J       Date:  2009-07-02       Impact factor: 11.598

6.  Antibody-mediated demyelination in experimental allergic encephalomyelitis is independent of complement membrane attack complex formation.

Authors:  S Piddlesden; H Lassmann; I Laffafian; B P Morgan; C Linington
Journal:  Clin Exp Immunol       Date:  1991-02       Impact factor: 4.330

7.  The sheep analogue of human CD59: purification and characterization of its complement inhibitory activity.

Authors:  C W van den Berg; R A Harrison; B P Morgan
Journal:  Immunology       Date:  1993-03       Impact factor: 7.397

8.  Molecular cloning and derived primary structure of cobra venom factor.

Authors:  D C Fritzinger; R Bredehorst; C W Vogel
Journal:  Proc Natl Acad Sci U S A       Date:  1994-12-20       Impact factor: 11.205

9.  Formation of C3-IgG complexes in serum by aggregated IgG and by non-immunoglobulin activators of complement.

Authors:  A P van Dam; C E Hack
Journal:  Immunology       Date:  1987-06       Impact factor: 7.397

10.  Increased local complement levels upon intraperitoneal injection of mice with Listeria monocytogenes and regulation by polyanions.

Authors:  J P Klerx; C J Beukelman; H van Dijk; F J van Overveld; W J van der Maaden; J M Willers
Journal:  Infect Immun       Date:  1985-09       Impact factor: 3.441
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