Literature DB >> 6491156

Monitoring protein conformational changes by quenching of intrinsic fluorescence.

G G Chang, H J Lee.   

Abstract

The quenching of intrinsic fluorescence of human serum albumin and pigeon liver malic enzyme by acrylamide was studied after the proteins were denatured to different stages. The progress of protein denaturation induced by guanidine hydrochloride was accompanied by increasing of effective dynamic quenching constant which provides a convenient parameter for monitoring protein conformational change.

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Year:  1984        PMID： 6491156     DOI： 10.1016/0165-022x(84)90019-8

Source DB:  PubMed          Journal:  J Biochem Biophys Methods        ISSN： 0165-022X


  7 in total

1.  Spectroscopic analyses of manganese ions effects on the conformational changes of inorganic pyrophosphatase from psychrophilic Shewanella sp. AS-11.

Authors:  Elvy Like Ginting; Chihiro Maeganeku; Hiroyuki Motoshima; Keiichi Watanabe
Journal:  Protein J       Date:  2014-02       Impact factor: 2.371

2.  Temperature-Induced Protein Conformational Changes in Barley Root Plasma Membrane-Enriched Microsomes: II. Intrinsic Protein Fluorescence.

Authors:  C R Caldwell
Journal:  Plant Physiol       Date:  1987-07       Impact factor: 8.340

3.  Inactivation of the endogenous argininosuccinate lyase activity of duck delta-crystallin by modification of an essential histidine residue with diethyl pyrocarbonate.

Authors:  H J Lee; S H Chiou; G G Chang
Journal:  Biochem J       Date:  1993-07-15       Impact factor: 3.857

4.  Interactions of nicotinamide-adenine dinucleotide phosphate analogues and fragments with pigeon liver malic enzyme. Synergistic effect between the nicotinamide and adenine moieties.

Authors:  H J Lee; G G Chang
Journal:  Biochem J       Date:  1987-07-15       Impact factor: 3.857

5.  In vitro study on the alterations of brain tubulin structure and assembly affected by magnetite nanoparticles.

Authors:  Ali Dadras; Gholam Hossein Riazi; Ali Afrasiabi; Ali Naghshineh; Behafarid Ghalandari; Farzad Mokhtari
Journal:  J Biol Inorg Chem       Date:  2013-02-09       Impact factor: 3.358

6.  The effects of anthracycline drugs on the conformational distribution of mouse P-glycoprotein explains their transport rate differences.

Authors:  P H Nguyen; K P Sigdel; K G Schaefer; G A K Mensah; G M King; A G Roberts
Journal:  Biochem Pharmacol       Date:  2020-01-16       Impact factor: 5.858

7.  Comparison of polymerization and structural behavior of microtubules in rat brain and sperm affected by the extremely low-frequency electromagnetic field.

Authors:  Dariush Gholami; Gholamhossein Riazi; Rouhollah Fathi; Mohsen Sharafi; Abdolhossein Shahverdi
Journal:  BMC Mol Cell Biol       Date:  2019-08-29
  7 in total

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