Literature DB >> 6490753

Use of high-performance liquid chromatography for preparing samples for microsequencing.

Y C Pan, J Wideman, R Blacher, M Chang, S Stein.   

Abstract

A method has been developed for the microsequencing of protein at subnanomole levels. The protein is carboxymethylated and freed of salts and reagents by reversed-phase chromatography prior to automated Edman degradation on a gas-phase sequencer. The carboxymethylated protein can also be fragmented chemically or enzymatically for further sequence analysis. The analytical techniques used to monitor the progress of the reactions all have picomole level sensitivity.

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Year:  1984        PMID： 6490753     DOI： 10.1016/s0021-9673(01)89024-5

Source DB:  PubMed          Journal:  J Chromatogr


  5 in total

1.  Rapid enzymatic degradation of growth hormone-releasing hormone by plasma in vitro and in vivo to a biologically inactive product cleaved at the NH2 terminus.

Authors:  L A Frohman; T R Downs; T C Williams; E P Heimer; Y C Pan; A M Felix
Journal:  J Clin Invest       Date:  1986-10       Impact factor: 14.808

2.  Identification of the probable site of choleragen-catalyzed ADP-ribosylation in a Go alpha-like protein based on cDNA sequence.

Authors:  C W Angus; K P Van Meurs; S C Tsai; R Adamik; M C Miedel; Y C Pan; H F Kung; J Moss; M Vaughan
Journal:  Proc Natl Acad Sci U S A       Date:  1986-08       Impact factor: 11.205

3.  Partial sequencing of human adult, human fetal, and bovine intestinal alkaline phosphatases: comparison with the human placental and liver isozymes.

Authors:  J C Hua; J Berger; Y C Pan; J D Hulmes; S Udenfriend
Journal:  Proc Natl Acad Sci U S A       Date:  1986-04       Impact factor: 11.205

4.  Dipeptidylpeptidase IV and trypsin-like enzymatic degradation of human growth hormone-releasing hormone in plasma.

Authors:  L A Frohman; T R Downs; E P Heimer; A M Felix
Journal:  J Clin Invest       Date:  1989-05       Impact factor: 14.808

5.  Aspartic acid-484 of nascent placental alkaline phosphatase condenses with a phosphatidylinositol glycan to become the carboxyl terminus of the mature enzyme.

Authors:  R Micanovic; C A Bailey; L Brink; L Gerber; Y C Pan; J D Hulmes; S Udenfriend
Journal:  Proc Natl Acad Sci U S A       Date:  1988-03       Impact factor: 11.205
  5 in total

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