| Literature DB >> 6488517 |
G Ellis, E P Diamandis, E E Giesbrecht, D Daneman, L C Allen.
Abstract
An automated "high-pressure" liquid-chromatographic assay for hemoglobin A1c is described. We use a 45-min incubation in acetate buffer (pH 5.5) to eliminate labile glycated hemoglobins. In this automated system conventional modules are used but it incorporates a solvent-switching valve to select either of two buffers, which differ in pH and NaCl concentration. The chromatographic column contains "polyCAT" (a weak cation-exchanger, polyaspartic acid linked to silica). Run time is 14 min per sample. The method is precise and results correlate well with those by other ion-exchange procedures.Entities:
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Year: 1984 PMID: 6488517
Source DB: PubMed Journal: Clin Chem ISSN: 0009-9147 Impact factor: 8.327