Literature DB >> 6486433

Strand separation of DNA fragments and their isolation from nondenaturing polyacrylamide gels.

R James, R A Bradshaw.   

Abstract

A technique for rapidly and quantitatively denaturing double-stranded DNA employing urea and moderate heat is described. The single DNA strands are resolved on high-percentage nondenaturing polyacrylamide gels from which they can be recovered for Maxam-Gilbert sequence analysis.

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Year:  1984        PMID： 6486433     DOI： 10.1016/0003-2697(84)90193-3

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  3 in total

1.  DNA Strand-Transfer Activity in Pea (Pisum sativum L.) Chloroplasts.

Authors:  H. Cerutti; A. T. Jagendorf
Journal:  Plant Physiol       Date:  1993-05       Impact factor: 8.340

2.  Single base mutation in the pro alpha 2(I) collagen gene that causes efficient splicing of RNA from exon 27 to exon 29 and synthesis of a shortened but in-frame pro alpha 2(I) chain.

Authors:  G Tromp; D J Prockop
Journal:  Proc Natl Acad Sci U S A       Date:  1988-07       Impact factor: 11.205

3.  Sequence analysis of the bovine coronavirus nucleocapsid and matrix protein genes.

Authors:  W Lapps; B G Hogue; D A Brian
Journal:  Virology       Date:  1987-03       Impact factor: 3.616
  3 in total

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