Literature DB >> 6482684

Squalene isolation by HPLC and quantitative comparison by HPLC and GLC.

J C Sulpice, J Férézou.   

Abstract

A new procedure is described for isolating and measuring squalene in plasma and in several organs of the rat. The unsaponifiable material was fractionated by normal phase HPLC on a silica gel column using a mobile phase consisting of hexane/propanol-2/water. The eluate was monitored at 215 nm. The squalene in the hydrocarbon fraction thus collected was than quantified on an analytical column eluted with hexane. Squalene concentrations ranging from 3 to 200 micrograms per ml of plasma or per g of fresh tissue were accurately measured. The results obtained agree with those of the squalene assays carried out by gas chromatography on a packed or capillary column.

Entities:  

Mesh：

Substances：

Year:  1984        PMID： 6482684     DOI： 10.1007/bf02534723

Source DB:  PubMed          Journal:  Lipids        ISSN： 0024-4201            Impact factor:   1.880


  8 in total

1.  Measurement of squalene in human tissues and plasma: validation and application.

Authors:  G C Liu; E H Ahrens; P H Schreibman; J R Crouse
Journal:  J Lipid Res       Date:  1976-01       Impact factor: 5.922

2.  Serum squalene and methyl sterols as indicators of cholesterol synthesis in vivo.

Authors:  T A Miettinen
Journal:  Life Sci       Date:  1969-07-15       Impact factor: 5.037

3.  Dietary squalene increases tissue sterols and fecal bile acids in the rat.

Authors:  R S Tilvis; T A Miettinen
Journal:  Lipids       Date:  1983-01       Impact factor: 1.880

4.  The separation of sterol intermediates in cholesterol biosynthesis by high pressure liquid chromatography.

Authors:  E Hansbury; T J Scallen
Journal:  J Lipid Res       Date:  1980-09       Impact factor: 5.922

5.  Plasma and lipoprotein cholesterol in rats fed L-amino acid-supplemented diets.

Authors:  C Sérougne; A Rukaj
Journal:  Ann Nutr Metab       Date:  1983       Impact factor: 3.374

6.  Liquid chromatographic analysis of sebum lipids and other lipids of medical interest.

Authors:  K Aitzetmüller; J Koch
Journal:  J Chromatogr       Date:  1978-03-01

7.  Squalene and sterol synthesis in isolated small-intestinal cells of the rat.

Authors:  T E Strandberg; R S Tilvis; T A Miettinen
Journal:  Scand J Gastroenterol       Date:  1981       Impact factor: 2.423

8.  Measurement of cholesterol synthesis in man by isotope kinetics of squalene.

Authors:  G C Liu; E H Ahrens; P H Schreibman; P Samuel; D J McNamara; J R Crouse
Journal:  Proc Natl Acad Sci U S A       Date:  1975-11       Impact factor: 11.205
  8 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.