Functional deterioration of mouse liver ribosomes during aging: translational activity and activity for formation of the 47 S initiation complex.

The translational activities of ribosomes from young and old mouse livers were examined in an assay system dependent on rabbit globin mRNA. Old ribosomes showed 30-40% lower activity than young ribosomes. This observation, together with our previous findings (N. Mori, D. Mizuno and S. Goto, Mech. Ageing Dev., 10 (1979) 379-398), suggests functional deterioration of ribosomes of old animals. To examine the mechanism of the deterioration, the activities for formation of the initiation complex of 40 S ribosomal subunits in the livers of young and old mice were examined in vitro. The activity was found to be 15-20% lower in old mice (21 months old or more) than in young ones. This fact partly explains the decreased activity for ribosomal protein synthesis in old mice.