Literature DB >> 6481339

A method for isolation of protoplasts from dermatophytes.

T Srikantha, G R Rao.   

Abstract

A method has been developed to isolate protoplasts from dermatophytes using Novozym 234. A simple technique of flotation in MgSO4 has been adapted to separate protoplasts from incubation mixture. Electron microscopic studies confirmed the absence of cell wall material on these protoplasts. The recovery of DNA from protoplasts was higher than from mycelia.

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Year:  1984        PMID： 6481339     DOI： 10.1099/00221287-130-6-1503

Source DB:  PubMed          Journal:  J Gen Microbiol        ISSN： 0022-1287


  3 in total

1.  Observation of protoplasts from the conidia of Arthrinium aureum by scanning electron microscopy.

Authors:  M Angeles Calvo; Montserrat Agut; Dolores Esqué
Journal:  Mycopathologia       Date:  2004-02       Impact factor: 2.574

2.  Optimization of protoplast formation, regeneration, and viability in Microsporum gypseum.

Authors:  M Chadegani; J J Brink; A Shehata; V Ahmadjian
Journal:  Mycopathologia       Date:  1989-07       Impact factor: 2.574

3.  Development of DNA probes for fingerprinting Aspergillus fumigatus.

Authors:  H Girardin; J P Latgé; T Srikantha; B Morrow; D R Soll
Journal:  J Clin Microbiol       Date:  1993-06       Impact factor: 5.948
  3 in total

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