Literature DB >> 6480219

Resolution of human mercapt- and nonmercaptalbumin by high-performance liquid chromatography.

M Sogami, S Nagoka, S Era, M Honda, K Noguchi.   

Abstract

Gel-exclusion high-performance liquid chromatographic (HPLC) analysis of human serum albumin (HSA) on PGP 2000 column (0.10 M sodium phosphate buffer, 0.30 M NaCl, pH 6.86) showed at least two peaks, the principal component corresponding to human mercaptalbumin (HMA) and the second one to human nonmercaptalbumin (HNA). Mechanism for the separation of HMA and HNA might be due to weak resin-HSA interaction. HPLC analysis of bovine plasma albumin (BPA) showed a single peak on PGP 2000 column. The elution volume of HSA was larger than that of BPA, resulting in a clear resolution of HSA and BPA.

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Year:  1984        PMID： 6480219     DOI： 10.1111/j.1399-3011.1984.tb00933.x

Source DB:  PubMed          Journal:  Int J Pept Protein Res        ISSN： 0367-8377


  15 in total

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4.  Validation of the chloramine-T induced oxidation of human serum albumin as a model for oxidative damage in vivo.

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