Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Continuous perfusion of mammalian cells embedded in agarose gel threads.

Literature DB >> 6479241

Continuous perfusion of mammalian cells embedded in agarose gel threads.

Abstract

A method for perfusing cells by embedding them in fine agarose gel threads is described and characterized. The rate of diffusion of a metabolite into the gel threads is determined by 31P-NMR spectroscopy. This perfusion method is shown to enable Chinese hamster lung fibroblasts (CHLF) to remain in a metabolically active state with high levels of intracellular ATP for many hours.

Entities: Chemical Species

Mesh：

Substances：

Year: 1984 PMID： 6479241 DOI： 10.1016/0014-4827(84)90176-9

Source DB: PubMed Journal: Exp Cell Res ISSN： 0014-4827 Impact factor: 3.905

Keyword Cloud
Cited

11 in total

1. Complete separation of intracellular and extracellular information in NMR spectra of perfused cells by diffusion-weighted spectroscopy.

Authors: P C Van Zijl; C T Moonen; P Faustino; J Pekar; O Kaplan; J S Cohen
Journal: Proc Natl Acad Sci U S A Date: 1991-04-15 Impact factor: 11.205

2. Competition between Li+ and Mg2+ in neuroblastoma SH-SY5Y cells: a fluorescence and 31P NMR study.

Authors: L Amari; B Layden; J Nikolakopoulos; Q Rong; D Mota de Freitas; G Baltazar; M M Castro; C F Geraldes
Journal: Biophys J Date: 1999-06 Impact factor: 4.033

3. Growth rate, labeling index, and radiation survival of cells grown in the Matrigel thread in vitro tumor model.

Authors: J J Casciari; L K Chin; J C Livesey; D Boyles; R G Steen; J S Rasey
Journal: In Vitro Cell Dev Biol Anim Date: 1995-09 Impact factor: 2.416

4. Real-Time In-Cell Nuclear Magnetic Resonance: Ribosome-Targeted Antibiotics Modulate Quinary Protein Interactions.

Authors: Leonard Breindel; Christopher DeMott; David S Burz; Alexander Shekhtman
Journal: Biochemistry Date: 2018-01-08 Impact factor: 3.162

5. Effect of ethanol on adenosine triphosphate, cytosolic free calcium, and cell injury in rat hepatocytes. Time course and effect of nutritional status.

Authors: A Gasbarrini; A B Borle; P Caraceni; A Colantoni; H Farghali; F Trevisani; M Bernardi; D H Van Thiel
Journal: Dig Dis Sci Date: 1996-11 Impact factor: 3.199

Continuous perfusion of mammalian cells embedded in agarose gel threads.

1. Complete separation of intracellular and extracellular information in NMR spectra of perfused cells by diffusion-weighted spectroscopy.

2. Competition between Li+ and Mg2+ in neuroblastoma SH-SY5Y cells: a fluorescence and 31P NMR study.

3. Growth rate, labeling index, and radiation survival of cells grown in the Matrigel thread in vitro tumor model.

4. Real-Time In-Cell Nuclear Magnetic Resonance: Ribosome-Targeted Antibiotics Modulate Quinary Protein Interactions.

5. Effect of ethanol on adenosine triphosphate, cytosolic free calcium, and cell injury in rat hepatocytes. Time course and effect of nutritional status.

Review 6. New advances in MR-compatible bioartificial liver.

Review 7. Metabolism of breast cancer cells as revealed by non-invasive magnetic resonance spectroscopy studies.

8. Hormone dependence of breast cancer cells and the effects of tamoxifen and estrogen: 31P NMR studies.

Review 9. Radio Signals from Live Cells: The Coming of Age of In-Cell Solution NMR.

10. Active metabolism unmasks functional protein-protein interactions in real time in-cell NMR.