Membrane disintegration and hemolysis of human erythrocytes by snake venom cardiotoxin (a membrane-disruptive polypeptide).

The action of 7.2 microM cardiotoxin on 0.25% (v/v) human erythrocytes in plasma extender solution was studied by osmotic fragility and hemolysis. The toxin loosened the membrane rigidity without lysis for about 25 min and then the cells hemolyzed for about 6 h at 37 degrees. The membrane was not perturbed at 15 degrees. The hemolysis of cells was optimal at 37 degrees and virtually stopped below 20 degrees. Both events could be suppressed by adding inorganic salts or toxin antibody. Once the membrane was loosened, the addition of phospholipase A2 would potentiate the disintegration of erythrocytes. In contrast, hemolysis of erythrocytes by (Lys)n lacked a non-lytic period and was temperature independent and insensitive towards salts and phospholipase A2.