Literature DB >> 6476895

Efficient extraction and sensitive radioimmunoassay for hCG in human urine.

T Shimizu, S Matsuura, M Mochizuki.   

Abstract

A single-step procedure for extracting and concentrating hCG in 24-h urine samples was developed. The procedure involves adsorption chromatography with Vitachange. The hCG recovery was more than 90% compared with about 51% for the standard kaolin-acetone method. Nonspecific urinary protein concentration of extracts was also reduced. Thus, this method for concentration of urinary hCG with subsequent radioimmunoassay (RIA) using an antiserum against the unique carboxylterminal peptide of hCG beta subunit provides a sensitive and specific method for detecting hCG in urine.

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Year:  1984        PMID： 6476895     DOI： 10.1007/bf02113809

Source DB:  PubMed          Journal:  Arch Gynecol        ISSN： 0170-9925


  12 in total

1.  The amino acid sequence of human chorionic gonadotropin. The alpha subunit and beta subunit.

Authors:  F J Morgan; S Birken; R E Canfield
Journal:  J Biol Chem       Date:  1975-07-10       Impact factor: 5.157

2.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

3.  Human chorionic gonadotropin. Amino acid sequence of the hormone-specific COOH-terminal region.

Authors:  H T Keutmann; R M Williams
Journal:  J Biol Chem       Date:  1977-08-10       Impact factor: 5.157

4.  Isolation and amino acid sequence of COOH-terminal fragments from the beta subunit of human choriogonadotropin.

Authors:  S Birken; R E Canfield
Journal:  J Biol Chem       Date:  1977-08-10       Impact factor: 5.157

Review 5.  Statistical quality control and routine data processing for radioimmunoassays and immunoradiometric assays.

Authors:  D Rodbard
Journal:  Clin Chem       Date:  1974-10       Impact factor: 8.327

6.  Studies on the apparent human chorionic gonadotropin-like factor in the crab Ovalipes ocellatus.

Authors:  T Maruo; S J Segal; S S Koide
Journal:  Endocrinology       Date:  1979-04       Impact factor: 4.736

7.  Enzymatic radioiodination of gonadotropins.

Authors:  Y Miyachi; J L Vaitukaitis; E Nieschlag; M B Lipsett
Journal:  J Clin Endocrinol Metab       Date:  1972-01       Impact factor: 5.958

8.  Highly sensitive radioimmunoassay for chorionic gonadotropin in human urine.

Authors:  A R Ayala; B C Nisula; H C Chen; G D Hodgen; G T Ross
Journal:  J Clin Endocrinol Metab       Date:  1978-10       Impact factor: 5.958

9.  A human chorionic gonadotropin-specific antiserum against synthetic peptide analogs to the carboxyl-terminal peptide of its beta-subunit.

Authors:  S Matsuura; M Ohashi; H C Chen; G D Hodgen
Journal:  Endocrinology       Date:  1979-02       Impact factor: 4.736

10.  Evidence for a gonadotropin from nonpregnant subjects that has physical, immunological, and biological similarities to human chorionic gonadotropin.

Authors:  H C Chen; G D Hodgen; S Matsuura; L J Lin; E Gross; L E Reichert; S Birken; R E Canfield; G T Ross
Journal:  Proc Natl Acad Sci U S A       Date:  1976-08       Impact factor: 11.205
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