| Literature DB >> 6476383 |
A J Cutler, F Constabel, W G Kurz, P D Shargool.
Abstract
A procedure for the quantitation of the delivery of liposome contents into Catharanthus roseus protoplasts has been developed. The method is based on the uptake of liposome encapsulated methylumbelliferyl beta-D-glucoside and its enzymatic hydrolysis to yield fluorescent methylumbelliferone. Since the free glucoside is not taken up by the protoplasts to a significant extent, the delivery of material in the nanomole range can be measured with ease.Entities:
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Year: 1984 PMID： 6476383 DOI： 10.1016/0003-2697(84)90038-1
Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365