| Literature DB >> 6473151 |
R Dimaline, J R Reeve, J E Shively, D Hawke.
Abstract
We have used gel filtration, ion exchange chromatography, affinity chromatography and reversed-phase HPLC to isolate vasoactive intestinal peptide from rat intestine. Microsequence analysis of 1 nmole peptide indicated that the sequence was identical to the porcine octacosapeptide VIP. In radioimmunoassay with four antisera and in the turkey pancreas bioassay, rat VIP was equipotent with highly purified preparations of porcine, human and canine VIP. A less basic rat VIP-variant was also isolated and the N-terminal decapeptide region that was sequenced was identical with that of porcine VIP.Entities:
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Year: 1984 PMID： 6473151 DOI： 10.1016/0196-9781(84)90204-3
Source DB: PubMed Journal: Peptides ISSN： 0196-9781 Impact factor: 3.750