The effect of membrane potential on initial velocity of GABA uptake and steady state distribution ratio in rat cortical synaptosomes.

Initial velocity of synaptosomal GABA uptake and steady state distribution ratio (DR) have been measured as a function of medium potassium concentration [( K]o) and in the presence of veratridine. Previous studies of the effect of [K]o and veratridine on membrane potential have been utilized to relate GABA uptake to membrane potential. Both initial velocity of uptake and steady state DR are functions of membrane potential; initial velocity and in DR are linearly related to membrane potential. Final DR is determined by the electrochemical potential gradient for the transported ions. Previous studies of the relation of [Na]o to uptake have shown that either one or two sodium ions may be translocated with each GABA molecule. Present results are consistent with this assumption if it is also assumed that an anion is co-transported with each GABA molecule.