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Literature DB >> 6472255

Chicken liver non-histone protein fraction repressing DNA transcription in vitro.

Abstract

A chicken liver non-histone protein fraction repressing DNA transcription in vitro was isolated using ultrasound shearing followed by precipitation with divalent cations. It was characterized by SDS-polyacrylamide electrophoresis and isoelectric focusing. This inhibitory protein fraction showed strong affinity to DNA and represented non-histone proteins with main bands of mol. wt about 35000 and 56000 and isoelectric points about 5.7 and 6.2. The inhibitory non-histone protein fraction seems to repress DNA transcription by interacting with DNA template rather than with RNA polymerase.

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Year: 1984 PMID： 6472255 DOI： 10.1007/bf00775148

Source DB: PubMed Journal: Mol Biol Rep ISSN： 0301-4851 Impact factor: 2.316

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References

30 in total

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Authors: M Gronow; G Griffiths
Journal: FEBS Lett Date: 1971-07-08 Impact factor: 4.124

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Authors: L J Kleinsmith; J Stein; G Stein
Journal: Proc Natl Acad Sci U S A Date: 1976-04 Impact factor: 11.205

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Authors: J F Chiu; Y H Tsai; K Sakuma; L S Hnilica
Journal: J Biol Chem Date: 1975-12-25 Impact factor: 5.157

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Authors: C S Teng; C T Teng; V G Allfrey
Journal: J Biol Chem Date: 1971-06-10 Impact factor: 5.157

Chicken liver non-histone protein fraction repressing DNA transcription in vitro.

1. Rapid isolation and separation of the non-histone proteins of rat liver nuclei.

2. Dephosphorylation of nonhistone proteins specifically alters the pattern of gene transcription in reconstituted chromatin.

3. Regulation of in vitro mRNA transcription by a fraction of chromosomal proteins.

4. Studies of nuclear acidic proteins. Evidence for their phosphorylation, tissue specificity, selective binding to deoxyribonucleic acid, and stimulation effects on transcription.

5. DNA dependent RNA polymerase from ehrlich ascites tumor cells. IV. A novel protein repressing RNA polymerase II.

6. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

7. Selective inhibition of transcription by a nonhistone protein isolated from Ehrlich ascites tumor chromatin.

8. Nonhistone chromosomal protein interaction with DNA/histone complexes. Transcription.

9. Regulation of transcription by DNA-bound non-histone nuclear proteins.

10. Small peptides controlling transcription in vitro are bound to chromatin DNA.