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Literature DB >> 6469718

A simple method for the fluorescence analysis of nucleic acid-dye complexes in cytological preparations.

A Villanueva, J C Stockert, R Armas-Portela.

Abstract

This communication describes a simple method for recording fluorescence emission spectra of cytological preparations using a conventional fluorescence spectrophotometer. The emission characteristics of "in situ" complexes between some basic fluorochromes (DAPI, 33258 Hoechst, acridine orange, pyronin Y, and ethidium bromide) and nucleic acid containing structures from smears of chicken blood and Ehrlich tumor cells (chromatin, basophilic cytoplasm) are briefly described.

Entities: Chemical Disease Species

Mesh：

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Year: 1984 PMID： 6469718 DOI： 10.1007/bf00495409

Source DB: PubMed Journal: Histochemistry ISSN： 0301-5564

21 in total

1. A new optical multichannel microspectrofluorometer.

Authors: M M Jotz; J E Gill; D T Davis
Journal: J Histochem Cytochem Date: 1976-01 Impact factor: 2.479

2. Microfluorometric detection of deoxyribonucleic acid replication in human metaphase chromosomes.

Authors: S A Latt
Journal: Proc Natl Acad Sci U S A Date: 1973-12 Impact factor: 11.205

3. Induced circular dichroism of DNA-dye complexes.

Authors: C H Lee; C T Chang; J G Wetmur
Journal: Biopolymers Date: 1973-05 Impact factor: 2.505

4. A microspectrofluorometer with epi-illumination operated under computer control.

Authors: J S Ploem; J A De Sterke; J Bonnet; H Wasmund
Journal: J Histochem Cytochem Date: 1974-07 Impact factor: 2.479

5. An evaluation of DNA fluorochromes, staining techniques, and analysis for flow cytometry. I. Unperturbed cell populations.

Authors: I W Taylor; B K Milthorpe
Journal: J Histochem Cytochem Date: 1980-11 Impact factor: 2.479

6. Fundamental aspects of the interaction of propidium diiodide with nuclei acids studied in a model system of polyacrylamide films.

Authors: J Tas; G Westerneng
Journal: J Histochem Cytochem Date: 1981-08 Impact factor: 2.479

A simple method for the fluorescence analysis of nucleic acid-dye complexes in cytological preparations.

1. A new optical multichannel microspectrofluorometer.

2. Microfluorometric detection of deoxyribonucleic acid replication in human metaphase chromosomes.

3. Induced circular dichroism of DNA-dye complexes.

4. A microspectrofluorometer with epi-illumination operated under computer control.

5. An evaluation of DNA fluorochromes, staining techniques, and analysis for flow cytometry. I. Unperturbed cell populations.

6. Fundamental aspects of the interaction of propidium diiodide with nuclei acids studied in a model system of polyacrylamide films.

7. Cytochemical mechanisms of chromatin fluorescence and staining reactions by thionin solutions.

8. Strategies for choosing a deoxyribonucleic acid stain for flow cytometry of metaphase chromosomes.

9. Four fluorochromes for the demonstration and microfluorometric estimation of RNA.

10. Flow cytometry of reticulocytes applied to clinical hematology.

1. Linear dichroism and polarized fluorescence of dye-complexed DNA fibers.