| Literature DB >> 6468584 |
V H Rao, I V Mongha, M R Ansari, N K Bhattacharyya.
Abstract
Frozen storage of rabbit embryos at the 16-cell stage in 2.0 M dimethylsulfoxide (DMSO) in phosphate-buffered saline (PBS) was achieved by a 2-step procedure. After storage for 10 days at -196 degrees C they were revived by rapidly thawing at 500 degrees C/min. On transfer of these embryos to pseudopregnant foster mothers, 50% survived to term. The difference in in vivo survival between frozen-thawed and frozen-thawed-cultured embryos was not significant.Entities:
Mesh：
Year: 1984 PMID： 6468584 DOI： 10.1007/bf01951970
Source DB: PubMed Journal: Experientia ISSN： 0014-4754