Literature DB >> 646824

A simple high-yield procedure for isolation of human urinary kallikreins.

N B Oza, J W Ryan.   

Abstract

Two human urinary kallikreins (fractions A-1 and A-2) were purified to apparently homogeneous forms. The two kallikreins were separated by affinity chromatography using Trasylol (aprotinin) covalently bound to Sepharose. The kallikreins were eluted with a pH gradient (pH 9.5-3.0). Fraction A-1 was eluted between pH 6.2 and 4.2 and fraction A-2 was eluted between pH 4.2 and 3.1. Final purification was obtained by chromatography on Sephacryl SS-200. Antibodies prepared against fraction A-2 were also reactive with fraction A-1; thus it may be possible to measure both by radioimmunoassay using the same antibody preparation.

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Year:  1978        PMID： 646824      PMCID： PMC1184161          DOI： 10.1042/bj1710285

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  12 in total

1.  A kallikrein-specific inhibitor in rat kidney tubules.

Authors:  R Geiger; K Mann
Journal:  Hoppe Seylers Z Physiol Chem       Date:  1976-04

2.  Synthesis of kallikreins by rat kidney slices.

Authors:  K Nustad; K Vaaje; J V Pierce
Journal:  Br J Pharmacol       Date:  1975-02       Impact factor: 8.739

3.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

4.  Isolation of rat urinary kallikrein and properties of its antibodies.

Authors:  N B Oza; V M Amin; R K McGregor; A G Scicli; O A Carretero
Journal:  Biochem Pharmacol       Date:  1976-07-15       Impact factor: 5.858

5.  Sensitive radiochemical esterolytic assays for urokinase.

Authors:  T Imanari; G M Wilcox; J J Pisano
Journal:  Clin Chim Acta       Date:  1976-09-06       Impact factor: 3.786

6.  Purification and properties of a human urinary kallikrein (kininogenase).

Authors:  V Hial; C R Diniz; M Mares-Guia
Journal:  Biochemistry       Date:  1974-10-08       Impact factor: 3.162

7.  A sensitive isotopic procedure for the assay of esterase activity: measurement of human urinary kallikrein.

Authors:  V H Beaven; J V Pierce; J J Pisano
Journal:  Clin Chim Acta       Date:  1971-03       Impact factor: 3.786

8.  Kallikrein-like activity in the urine of renal hypertensive rats.

Authors:  H R Croxatto; M San Martin
Journal:  Experientia       Date:  1970-11-15

9.  Purification of rat urinary kallikreins and their specific antibody.

Authors:  K Nustad; J V Pierce
Journal:  Biochemistry       Date:  1974-05-21       Impact factor: 3.162

10.  Relationships among urinary kallikrein, mineralocorticoids and human hypertensive disease.

Authors:  H S Margolius; D Horwitz; J J Pisano; H R Keiser
Journal:  Fed Proc       Date:  1976-02
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  3 in total

1.  Differences in the chemical and catalytic characteristics of two crystallographically 'identical' enzyme catalytic sites. Characterization of actinidin and papain by a combination of pH-dependent substrate catalysis kinetics and reactivity probe studies targeted on the catalytic-site thiol group and its immediate microenvironment.

Authors:  E Salih; J P Malthouse; D Kowlessur; M Jarvis; M O'Driscoll; K Brocklehurst
Journal:  Biochem J       Date:  1987-10-01       Impact factor: 3.857

2.  Initiation of plasma prorenin activation by Hageman factor-dependent conversion of plasma prekallikrein to kallikrein.

Authors:  J E Sealey; S A Atlas; J H Laragh; M Silverberg; A P Kaplan
Journal:  Proc Natl Acad Sci U S A       Date:  1979-11       Impact factor: 11.205

3.  Structural studies of human urinary kallikrein (urokallikrein).

Authors:  O K ole-MoiYoi; J Spragg; K F Austen
Journal:  Proc Natl Acad Sci U S A       Date:  1979-07       Impact factor: 11.205
  3 in total

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