Literature DB >> 6463590

Preparative isolation of immune complexes from serum by sucrose gradient ultracentrifugation.

E Rødahl, O J Iversen, A B Dalen.   

Abstract

Immune complexes were isolated by ultracentrifugation in sucrose gradients (20-65% (w/w]. the centrifugation procedure was demonstrated to be isopycnic. The banding density of the complexes was influenced by the chemical nature and molecular size of the antigen and by the antigen to antibody ratio. The method was applied for preparative isolation of immune complexes from patients with systemic lupus erythematosus, rheumatoid arthritis, ankylosing spondylitis, psoriatic arthritis, and uncomplicated psoriasis.

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Year:  1984        PMID： 6463590     DOI： 10.1111/j.1365-3083.1984.tb00973.x

Source DB:  PubMed          Journal:  Scand J Immunol        ISSN： 0300-9475            Impact factor:   3.487


  5 in total

1.  Combining ultracentrifugation and peptide termini group-specific immunoprecipitation for multiplex plasma protein analysis.

Authors:  Sonja Volk; Thomas D Schreiber; David Eisen; Calvin Wiese; Hannes Planatscher; Christopher J Pynn; Dieter Stoll; Markus F Templin; Thomas O Joos; Oliver Pötz
Journal:  Mol Cell Proteomics       Date:  2012-04-23       Impact factor: 5.911

2.  Ligation of Fc gamma receptor IIB inhibits antibody-dependent enhancement of dengue virus infection.

Authors:  Kuan Rong Chan; Summer Li-Xin Zhang; Hwee Cheng Tan; Ying Kai Chan; Angelia Chow; Angeline Pei Chiew Lim; Subhash G Vasudevan; Brendon J Hanson; Eng Eong Ooi
Journal:  Proc Natl Acad Sci U S A       Date:  2011-07-11       Impact factor: 11.205

3.  Analysis of circulating immune complexes from patients with ankylosing spondylitis by gel electrophoresis and immunoblotting using antiserum against a psoriasis associated retrovirus-like particle.

Authors:  E Rødahl; O J Iversen
Journal:  Ann Rheum Dis       Date:  1986-11       Impact factor: 19.103

4.  Participation of antigens related to the psoriasis associated antigen, pso p27, in immune complex formation in patients with ankylosing spondylitis.

Authors:  E Rødahl; K Asbakk; O J Iversen
Journal:  Ann Rheum Dis       Date:  1988-08       Impact factor: 19.103

5.  The major internal protein, p27, of a retrovirus-like particle participates in immune complex formation in psoriasis.

Authors:  O J Iversen; E Rødahl
Journal:  Arch Virol       Date:  1985       Impact factor: 2.574
  5 in total

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