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Literature DB >> 6461444

Delineation of human thymocyte differentiation pathways utilizing double-staining techniques with monoclonal antibodies.

N Tidman, G Janossy, M Bodger, S Granger, P C Kung, G Goldstein.

Abstract

Three new techniques have been introduced for the phenotypic analysis of human thymocytes and T cells. Monoclonal antibodies of different subclasses (i.e. IgG1 and IgG2) were used in various combinations and labelled with subclass-specific second layers conjugated to different fluorochromes. Tissue sections were studied with combinations of monoclonal and conventional reagents, and anti-mouse Ig-coated macrobead columns were used to separate antibody-tagged cells. With the help of these simple techniques, four stage of thymocyte differentiation (prothymocyte, cortical, intermediate and medullary cell types) can be described in detail and the microanatomical relation of T cell subsets with other cell types studied.

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Year: 1981 PMID： 6461444 PMCID： PMC1537423

Source DB: PubMed Journal: Clin Exp Immunol ISSN： 0009-9104 Impact factor: 4.330

16 in total

1. Monoclonal antibodies defining distinctive human T cell surface antigens.

Authors: P Kung; G Goldstein; E L Reinherz; S F Schlossman
Journal: Science Date: 1979-10-19 Impact factor: 47.728

2. The human thymic microenvironment: an immunohistologic study.

Authors: G Janossy; J A Thomas; F J Bollum; S Granger; G Pizzolo; K F Bradstock; L Wong; A McMichael; K Ganeshaguru; A V Hoffbrand
Journal: J Immunol Date: 1980-07 Impact factor: 5.422

3. Acute lymphoblastic leukaemia associated antigen. IV. Expression on non-leukaemic 'lymphoid' cells.

Authors: M Greaves; D Delia; G Janossy; N Rapson; J Chessells; M Woods; G Prentice
Journal: Leuk Res Date: 1980 Impact factor: 3.156

4. Terminal transferase-positive human bone marrow cells exhibit the antigenic phenotype of common acute lymphoblastic leukemia.

Authors: G Janossy; F J Bollum; K F Bradstock; A McMichael; N Rapson; M F Greaves
Journal: J Immunol Date: 1979-10 Impact factor: 5.422

5. Antibody to terminal deoxynucleotidyl transferase.

Authors: F J Bollum
Journal: Proc Natl Acad Sci U S A Date: 1975-10 Impact factor: 11.205

6. A human thymocyte antigen defined by a hybrid myeloma monoclonal antibody.

Authors: A J McMichael; J R Pilch; G Galfré; D Y Mason; J W Fabre; C Milstein
Journal: Eur J Immunol Date: 1979-03 Impact factor: 5.532

7. Distribution of t lymphocyte subsets in the human bone marrow and thymus: an analysis with monoclonal antibodies.

Authors: G Janossy; N Tidman; E S Papageorgiou; P C Kung; G Goldstein
Journal: J Immunol Date: 1981-04 Impact factor: 5.422

8. Discrete stages of human intrathymic differentiation: analysis of normal thymocytes and leukemic lymphoblasts of T-cell lineage.

Authors: E L Reinherz; P C Kung; G Goldstein; R H Levey; S F Schlossman
Journal: Proc Natl Acad Sci U S A Date: 1980-03 Impact factor: 11.205

9. Cellular phenotypes of normal and leukemic hemopoietic cells determined by analysis with selected antibody combinations.

Authors: G Janossy; F J Bollum; K F Bradstock; J Ashley
Journal: Blood Date: 1980-09 Impact factor: 22.113

10. Subpopulations of normal and leukemic human thymocytes: an analysis with the use of monoclonal antibodies.

Authors: K F Bradstock; G Janossy; G Pizzolo; A V Hoffbrand; A McMichael; J R Pilch; C Milstein; P Beverley; F J Bollum
Journal: J Natl Cancer Inst Date: 1980-07 Impact factor: 13.506

30 in total

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Authors: Chris M van der Loos
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2. Chromogen-based immunohistochemical method for elucidation of the coexpression of two antigens using antibodies from the same species.

Authors: Takahiro Nakata; Natsuki Suzuki
Journal: J Histochem Cytochem Date: 2012-05-19 Impact factor: 2.479

3. Stain-Decolorize-Stain (SDS): a new technique for multiple staining.

Authors: Jing Li; Yan Zhou; Jiang Gu
Journal: Histochem Cell Biol Date: 2014-01-05 Impact factor: 4.304

Review 4. Practical suggestions for successful immunoenzyme double-staining experiments.

Authors: C M van der Loos; A E Becker; J J van den Oord
Journal: Histochem J Date: 1993-01

5. Use of commercially available monoclonal antibodies for immunoenzyme double staining.

Authors: C M van der Loos; J J van den Oord; P K Das; H J Houthoff
Journal: Histochem J Date: 1988-08

6. Coincident change of cellular function and phenotype in the course of a suppressor T cell acute lymphocytic leukaemia.

Authors: G Sieber; W D Ludwig; H Teichmann; F Herrmann; H Rühl
Journal: Clin Exp Immunol Date: 1985-06 Impact factor: 4.330

7. The microenvironment of coeliac disease: T cell phenotypes and expression of the T2 'T blast' antigen by small bowel lymphocytes.

Authors: G Malizia; L K Trejdosiewicz; G M Wood; P D Howdle; G Janossy; M S Losowsky
Journal: Clin Exp Immunol Date: 1985-05 Impact factor: 4.330