Literature DB >> 6460917

Liposome-mediated genetic transformation of Neurospora crassa.

A Radford, S Pope, A Sazci, M J Fraser, J H Parish.   

Abstract

Transformation of Neurospora crassa spheroplasts is reported for three different genes, using uncloned Neurospora DNA, both naked and encapsulated in synthetic phosphatidylserine liposomes. Whereas transformation by naked DNA is DNase-sensitive, that by liposomes is not. Per unit of transforming DNA, liposome transformation is significantly more efficient than that with naked DNA, ranging from 19x for the am gene to 41x for pyr-3. Levels of activity of pyr-3 and am transformants, and segregation data on pyr-3 transformation are given.

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Year:  1981        PMID： 6460917     DOI： 10.1007/bf00352544

Source DB:  PubMed          Journal:  Mol Gen Genet        ISSN： 0026-8925


  9 in total

1.  Isolation of spheroplast and membrane vesicles from yeast and filamentous fungi.

Authors:  W R Wiley
Journal:  Methods Enzymol       Date:  1974       Impact factor: 1.600

2.  Amplification and characterization of a beta-globin gene synthesized in vitro.

Authors:  T Maniatis; S G Kee; A Efstratiadis; F C Kafatos
Journal:  Cell       Date:  1976-06       Impact factor: 41.582

3.  Multiple active varieties of Neurospora glutamate dehydrogenase formed by hybridization between two inactive mutant proteins in vivo and in vitro.

Authors:  A Coddington; J R Fincham; T K Sundaram
Journal:  J Mol Biol       Date:  1966-06       Impact factor: 5.469

4.  High-frequency transformation of yeast: autonomous replication of hybrid DNA molecules.

Authors:  K Struhl; D T Stinchcomb; S Scherer; R W Davis
Journal:  Proc Natl Acad Sci U S A       Date:  1979-03       Impact factor: 11.205

5.  Transformation of yeast by a replicating hybrid plasmid.

Authors:  J D Beggs
Journal:  Nature       Date:  1978-09-14       Impact factor: 49.962

6.  Introduction of liposome-encapsulated SV40 DNA into cells.

Authors:  R Fraley; S Subramani; P Berg; D Papahadjopoulos
Journal:  J Biol Chem       Date:  1980-11-10       Impact factor: 5.157

7.  Non-Mendelian inheritance of DNA-induced inositol independence in Neurospora.

Authors:  N C Mishra; E L Tatum
Journal:  Proc Natl Acad Sci U S A       Date:  1973-12       Impact factor: 11.205

8.  Efficient transformation of Neurospora crassa by utilizing hybrid plasmid DNA.

Authors:  M E Case; M Schweizer; S R Kushner; N H Giles
Journal:  Proc Natl Acad Sci U S A       Date:  1979-10       Impact factor: 11.205

9.  Entrapment of a bacterial plasmid in phospholipid vesicles: potential for gene transfer.

Authors:  R T Fraley; C S Fornari; S Kaplan
Journal:  Proc Natl Acad Sci U S A       Date:  1979-07       Impact factor: 11.205
  9 in total
  3 in total

1.  Transformation of Ustilago maydis by a plasmid containing yeast 2-micron DNA.

Authors:  G R Banks
Journal:  Curr Genet       Date:  1983-03       Impact factor: 3.886

Review 2.  Transformation in fungi.

Authors:  J R Fincham
Journal:  Microbiol Rev       Date:  1989-03

3.  Diverse data supports the transition of filamentous fungal model organisms into the post-genomics era.

Authors:  Kevin McCluskey; Scott E Baker
Journal:  Mycology       Date:  2017-02-17
  3 in total

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