Effects of exogenous insoluble glucan primer on insoluble glucan synthesis by Streptococcus mutans.

The effects of exogenous glucans on WIG synthesis by S. mutans were investigated by turbidimetric measurement. The rapid synthesis of WIG by an enzyme preparation from PS-14 culture fluid proceeded after a lag period of several min. The addition of exogenous WIG, which was previously prepared using the PS-14 enzyme, caused a marked reduction of the lag period with little change in the reaction rate. The length of lag period was affected by the concentrations of WIG primer, enzyme and salts, and the reaction pH. The WIGs from other strains and the periodate-treated WIGs also exhibited effects similar to the PS-14 glucan. In contrast, dextran, starch, dextrin, laminarin, cellulose, chitin, and inulin were without any effect. These results suggest that such lag-reducing effect is specific to glucans containing a significant amount of alpha-(1 leads to 3) linkages.