| Literature DB >> 6455432 |
D W Kufe, P P Major, E M Egan, E Loh.
Abstract
We have employed cesium sulfate density gradient centrifugation to separate RNA and DNA of L1210 cells labeled with [3H]fluorodeoxyuridine. We have analyzed nucleotide and nucleoside digests of purified DNA from the [3H]fluorodeoxyuridine-labeled cells and demonstrate by reverse phase and anion exchange high pressure liquid chromatography the presence of tritium radioactivity co-migrating with fluorodeoxyuridine 5'-monophosphate or fluorodeoxyuridine. These observations demonstrate the internucleotide incorporation of fluorodeoxyuridine in DNA and suggest a new mechanism of action for this cytotoxic and mutagenic agent.Entities:
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Year: 1981 PMID： 6455432
Source DB: PubMed Journal: J Biol Chem ISSN： 0021-9258 Impact factor: 5.157