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Literature DB >> 6452630

Cloning and complete nucleotide sequence of human 5'-alpha-globin gene.

Abstract

We have cloned one of the two human alpha-globin genes and report its complete nucleotide sequence. The gene is 832 base pairs (bp) long from the 5'-cap site to the 3'-polyadenylylation site. The amino acid coding sequences are separated into three segments (exons) by two short (117 and 140 bp) intervening sequences. Highly conserved regions are identified in the 5'-flanking region, intron-exon junctions, and 3' noncoding regions that may have functional significance.

Entities: Gene Species

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Year: 1980 PMID： 6452630 PMCID： PMC350439 DOI： 10.1073/pnas.77.12.7054

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

42 in total

1. Nucleotide sequence of an RNA polymerase binding site at an early T7 promoter.

Authors: D Pribnow
Journal: Proc Natl Acad Sci U S A Date: 1975-03 Impact factor: 11.205

2. Preparative fractionation of DNA restriction fragments by reversed phase column chromatography.

Authors: S C Hardies; R D Wells
Journal: Proc Natl Acad Sci U S A Date: 1976-09 Impact factor: 11.205

3. Improved derivative of a phage lambda EK2 vector for cloning recombinant DNA.

Authors: D Tiemer; L Enquist; P Leder
Journal: Nature Date: 1976-10-07 Impact factor: 49.962

4. Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors: E M Southern
Journal: J Mol Biol Date: 1975-11-05 Impact factor: 5.469

5. 3' non-coding region sequences in eukaryotic messenger RNA.

Authors: N J Proudfoot; G G Brownlee
Journal: Nature Date: 1976-09-16 Impact factor: 49.962

6. Isolation of high-molecular-weight DNA from mammalian cells.

Authors: M Gross-Bellard; P Oudet; P Chambon
Journal: Eur J Biochem Date: 1973-07-02

7. Sequence of promoter for coat protein gene of bacteriophage fd.

Authors: M Takanami; K Sugimoto; H Sugisaki; T Okamoto
Journal: Nature Date: 1976-03-25 Impact factor: 49.962

8. Resolution of multiple ribonucleic acid species by polyacrylamide gel electrophoresis.

Authors: A C Peacock; C W Dingman
Journal: Biochemistry Date: 1967-06 Impact factor: 3.162

9. Nucleotide sequence of an RNA polymerase binding site from the DNA of bacteriophage fd.

Authors: H Schaller; C Gray; K Herrmann
Journal: Proc Natl Acad Sci U S A Date: 1975-02 Impact factor: 11.205

10. Nucleotide sequence of the rightward operator of phage lambda.

Authors: T Maniatis; A Jeffrey; D G Kleid
Journal: Proc Natl Acad Sci U S A Date: 1975-03 Impact factor: 11.205

45 in total

1. Two upstream elements activate transcription of a major histocompatibility complex class I gene in vitro.

Authors: P H Driggers; B A Elenbaas; J B An; I J Lee; K Ozato
Journal: Nucleic Acids Res Date: 1992-05-25 Impact factor: 16.971

2. Modification of the melting properties of duplex DNA by attachment of a GC-rich DNA sequence as determined by denaturing gradient gel electrophoresis.

Authors: R M Myers; S G Fischer; T Maniatis; L S Lerman
Journal: Nucleic Acids Res Date: 1985-05-10 Impact factor: 16.971

3. Hemoglobin M Iwate is caused by a C----T transition in codon 87 of the human alpha 1-globin gene.

Authors: J Horst; G Assum; E U Griese; A Eigel; W Hampl; E Kohne
Journal: Hum Genet Date: 1987-01 Impact factor: 4.132

4. Anomalous and selective DNA mutations of the Old World monkey alpha-globin genes.

Authors: J P Shaw; J Marks; C C Shen; C K Shen
Journal: Proc Natl Acad Sci U S A Date: 1989-02 Impact factor: 11.205

5. The orangutan adult alpha-globin gene locus: duplicated functional genes and a newly detected member of the primate alpha-globin gene family.

Authors: J Marks; J P Shaw; C K Shen
Journal: Proc Natl Acad Sci U S A Date: 1986-03 Impact factor: 11.205

6. Identification of estrogen-responsive DNA sequences by transient expression experiments in a human breast cancer cell line.

Authors: A Seiler-Tuyns; P Walker; E Martinez; A M Mérillat; F Givel; W Wahli
Journal: Nucleic Acids Res Date: 1986-11-25 Impact factor: 16.971

10. Compensatory increase in alpha 1-globin gene expression in individuals heterozygous for the alpha-thalassemia-2 deletion.

Authors: S A Liebhaber; F E Cash; D M Main
Journal: J Clin Invest Date: 1985-09 Impact factor: 14.808