Photolabelling with 8-azido-adenine nucleotides of adenine nucleotide-binding sites in isolated spinach chloroplast ATPase (CF1).

1. Photolabelling of chloroplast ATPase (CF1) with either 8-azido-ATP or 8-azido-ADP leads to inactivation of the ATPase activity. ATP and ADP protect against the inactivation, whereas AMP dose not. 2. Ca2+ has little if any effect on the degree of inactivation by photolabelling with 8-azido-ADP, but, at the same degree of inactivation, twice as much label is bound in the presence of Ca2+ as in its absence. 3. The degree of inactivation of ATPase and the amount of bound photolabel are independent of the extent of pre-activation of the CF1. 4. Upon extrapolation to complete inactivation, 2 mol label, either 8-azido-ATP or 8-azido-ADP can be bound. 5. In all cases the label is bound specifically to the alpha and beta subunits in almost equal amounts. The location of the bound label is not affected by addition of Ca2+, ATP or ADP.