| Literature DB >> 6449636 |
Abstract
A simple semi-automated method for the determination of erythrocyte plasma membrane (Mg2+)-ATPase, (Na+ + K+ + Mg2+)-ATPase, and the (Ca2+ + Mg2+)-ATPase is described in detail. A nonautomated ATPase enzyme assay for multiple membrane ATPases and automated methods for determination of inorganic phosphate as well as membrane-bound protein are described. Together, the methods represent a system that has the flexibility and sensitivity for basic research applications and the capacity to process relatively large numbers of samples as is common in clinical laboratory screening.Entities:
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Year: 1980 PMID: 6449636 DOI: 10.1016/0160-5402(80)90019-4
Source DB: PubMed Journal: J Pharmacol Methods ISSN: 0160-5402