Literature DB >> 6449636

A semi-automated method for the determination of multiple membrane ATPase activities.

B U Raess, F F Vincenzi.   

Abstract

A simple semi-automated method for the determination of erythrocyte plasma membrane (Mg2+)-ATPase, (Na+ + K+ + Mg2+)-ATPase, and the (Ca2+ + Mg2+)-ATPase is described in detail. A nonautomated ATPase enzyme assay for multiple membrane ATPases and automated methods for determination of inorganic phosphate as well as membrane-bound protein are described. Together, the methods represent a system that has the flexibility and sensitivity for basic research applications and the capacity to process relatively large numbers of samples as is common in clinical laboratory screening.

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Year:  1980        PMID: 6449636     DOI: 10.1016/0160-5402(80)90019-4

Source DB:  PubMed          Journal:  J Pharmacol Methods        ISSN: 0160-5402


  11 in total

1.  Structure--function relationship of the human erythrocyte plasma membrane Ca(2+)-ATPase revealed by V8 protease treatment.

Authors:  K K Wang; B D Roufogalis; T H Kuo
Journal:  Biochem J       Date:  1991-10-15       Impact factor: 3.857

2.  Reduction of erythrocyte (Na+-K+)ATPase activity in type 1 (insulin-dependent) diabetic subjects and its activation by homologous plasma.

Authors:  P Finotti; P Palatini
Journal:  Diabetologia       Date:  1986-09       Impact factor: 10.122

3.  Proton countertransport by the reconstituted erythrocyte Ca2+-translocating ATPase: evidence using ionophoretic compounds.

Authors:  A Villalobo; B D Roufogalis
Journal:  J Membr Biol       Date:  1986       Impact factor: 1.843

4.  Age- and sex-related differences in nuclear lipid content and nucleoside triphosphatase activity in the JCR:LA-cp corpulent rat.

Authors:  M P Czubryt; J C Russell; J Sarantopoulos; J S Gilchrist; G N Pierce
Journal:  Mol Cell Biochem       Date:  1997-11       Impact factor: 3.396

5.  Characterization of a new plasma membrane-associated ecto-5'-phosphodiesterase/nucleotide-pyrophosphatase from rat hepatocarcinoma AS-30D cells.

Authors:  R M García-Nieto; E S José; J Martín-Nieto; A Villalobo
Journal:  J Physiol Biochem       Date:  2001-03       Impact factor: 4.158

6.  Calpain I activates Ca2+ transport by the reconstituted erythrocyte Ca2+ pump.

Authors:  K K Wang; B D Roufogalis; A Villalobo
Journal:  J Membr Biol       Date:  1989-12       Impact factor: 1.843

7.  Activation of erythrocyte Ca2+-plus-Mg2+-stimulated adenosine triphosphatase by protein kinase (cyclic AMP-dependent) inhibitor. Comparison with calmodulin.

Authors:  A V Minocherhomjee; B D Roufogalis
Journal:  Biochem J       Date:  1982-09-15       Impact factor: 3.857

8.  Plasma membrane Ca2+ transport: antagonism by several potential inhibitors.

Authors:  T R Hinds; B U Raess; F F Vincenzi
Journal:  J Membr Biol       Date:  1981-01-30       Impact factor: 1.843

9.  Protective effect of taurine on hypochlorous acid toxicity to nuclear nucleoside triphosphatase in isolated nuclei from rat liver.

Authors:  Ju-Xiang Li; Yong-Zheng Pang; Chao-Shu Tang; Zai-Quan Li
Journal:  World J Gastroenterol       Date:  2004-03-01       Impact factor: 5.742

10.  Presence of a high-affinity Ca2+- and Mg2+-dependent ATPase in rat peritoneal mast-cell membranes.

Authors:  L M Amende; M A Donlon
Journal:  Biochem J       Date:  1985-02-15       Impact factor: 3.857

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