Cholesteryl glucoside in Candida bogoriensis.

Extraction of lipids with CHCl3/CH3OH/1 M HCl from fresh and frozen cells of Candida bogoriensis showed the presence of a steryl glucoside. The product was purified and identified as a beta-cholesteryl glucoside. It hydrolyzes in methanolic HCl and therefore it is a steryl glucoside. The quantitation of the extracted cholesteryl glucoside was pursued with high-pressure liquid chromatography. The chemical ionization mass spectra of the isolated and the synthesized beta-cholesteryl glucosides were compared and found identical. The cell wall of Candida bogoriensis was weakened with enzyme (glusulase) and after homogenisation and sucrose density gradient centrifugation, five layers separated. Only the pellets of one layer showed the presence of cholesteryl glucoside in thin-layer chromatography after extraction with solvents. In the same layer, the activity of sterol glucosyl transferase was found.