Activation of murine T-suppressor lymphocytes by group A streptococcal and staphylococcal pyurogenic exotoxins.

The effect of group A streptococcal pyrogenic exotoxin (PE) type C and staphylococcal PE on the in vitro antibody response to sheep erythrocytes was studied in cultures of mouse spleen cells. Both exotoxins suppressed the day 4 direct plaque-forming cell response when added to the cultures. The maximum suppression was obtained with 1.0 or 0.1 ng of toxin per culture, and the suppressive effect was reversed by addition of gangliosides to the cultures at the same time as the exotoxins. Preincubation of T lymphocytes for 4 days with either exotoxin resulted in the generation of a suppressor cell population, which produced dose-dependent suppression of the direct plaque-forming cell response when added to fresh sheep eyrthrocyte-activated splenocytes. The suppression obtained was not reversed by gangliosides indicating toxin carry-over was not responsible for the effect. B cells, preincubated with exotoxin, failed to suppress the direct plaque-forming cell response of fresh erythrocyte-activated spleen cells.